Localization of [3H]-imipramine binding sites in rat brain by light microscopic autoradiography.
Localization of [3H]-imipramine binding sites in rat brain by light microscopic autoradiography.
复制标题
通过光学显微镜放射自显影定位大鼠脑中的[3H]-丙咪嗪结合位点。
DOI：
10.1016/0024-3205(83)90766-x
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发表时间：
1983
期刊：
影响因子：
6.1
通讯作者：
J. Wamsley
中科院分区：
文献类型：
--
作者：
K. Grabowsky;R. Mccabe;J. Wamsley
研究了[3H]-丙咪嗪在大鼠前脑载玻片组织切片中的结合特性，以确定在放射自显影定位之前标记位点的最佳结合条件。实验条件和丙咪嗪结合动力学与膜制剂中用于初步确定丙咪嗪结合位点的条件相当吻合。随后使用这些参数对切片进行标记，允许在大脑皮层、纹状体、几个边缘和视觉系统结构等区域进行高浓度丙咪嗪结合位点的放射自显像定位。此外，在显示丙咪嗪结合的区域和已知由血清素能神经元支配的区域之间有明显的重叠。这项研究概述了丙咪嗪在大脑中的潜在作用部位，并确定了未来研究的区域，这些研究试图定位与抑郁症病因有关的大脑区域和与抗抑郁药物治疗副作用有关的大脑区域。
The binding characteristics of [3H]-imipramine in slide mounted tissue sections of rat forebrain have been studied to ascertain the optimal binding conditions for labeling the sites prior to autoradiographic localization. The conditions for the experiments and the kinetics of the imipramine binding correspond reasonably well with those used in membrane preparations to initially define the imipramine binding site. Subsequent labeling of sections, using these parameters, allowed the autoradiographic localization of high concentrations of imipramine binding sites in such areas as the cerebral cortex, striatum, and several limbic and visual system structures. In addition, there was a marked overlap between regions demonstrating imipramine binding and areas known to be innervated by serotonergic neurons. This study outlines the potential sites of action of imipramine in the brain and defines areas for future investigations which attempt to localize brain regions involved in the etiology of depression and areas involved in the side effects of antidepressant drug therapy.
影响因子：
--
作者：
D. Charney;D. Menkes;G. Heninger
通讯作者：
D. Charney;D. Menkes;G. Heninger
[3H]Mianserin：大鼠脑中血清素和组胺受体的差异标记。
DOI：
--
发表时间：
1981
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Peroutka,SJ;Snyder,SH
通讯作者：
Snyder,SH