Developmental analyses of divarications in leaves of an aquatic fern Microsorum pteropus and its varieties
Developmental analyses of divarications in leaves of an aquatic fern Microsorum pteropus and its varieties
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DOI：
10.1371/journal.pone.0210141
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发表时间：
2019-01
期刊：
影响因子：
3.7
通讯作者：
Saori Miyoshi;S. Kimura;Ryo Ootsuki;T. Higaki;Akiko M. Nakamasu
中科院分区：
文献类型：
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作者：
Saori Miyoshi;S. Kimura;Ryo Ootsuki;T. Higaki;Akiko M. Nakamasu
植物的叶子形状各异。在早期生长过程中形成的分叉模式是决定叶子形状的关键因素之一。我们利用叶的Microsorum pteropus，半水生蕨类植物，和密切相关的品种来分析分歧模式的变化。叶表现出三种主要类型的分叉：无裂片、分叉和三叉（即，单轴分枝）。我们的调查，他们的发展过程中，使用延时成像，发现本地化的增长和解剖叶片附近的每个叶尖。使用EdU标记测定的脉冲追踪策略证实了负责顶端生长的受限细胞分裂。
Plant leaves occur in diverse shapes. Divarication patterns that develop during early growths are one of key factors that determine leaf shapes. We utilized leaves of Microsorum pteropus, a semi-aquatic fern, and closely related varieties to analyze a variation in the divarication patterns. The leaves exhibited three major types of divarication: no lobes, bifurcation, and trifurcation (i.e., monopodial branching). Our investigation of their developmental processes, using time-lapse imaging, revealed localized growths and dissections of blades near each leaf apex. Restricted cell divisions responsible for the apical growths were confirmed using a pulse-chase strategy for EdU labeling assays.