Crosstalk in the responses to abiotic and biotic stresses in Arabidopsis:: Analysis of gene expression in cytochrome P450 gene superfamily by cDNA microarray
Crosstalk in the responses to abiotic and biotic stresses in Arabidopsis:: Analysis of gene expression in cytochrome P450 gene superfamily by cDNA microarray
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DOI：
10.1007/s11103-004-0685-1
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发表时间：
2004-05-01
影响因子：
5.1
通讯作者：
Shinozaki, K
中科院分区：
文献类型：
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作者：
Narusaka, Y;Narusaka, M;Shinozaki, K
从拟南芥全长cDNA文库中，我们收集了ca. 7000（7 K）个独立的全长cDNA以制备cDNA微阵列。7 K cDNA文库包含49个细胞色素P450基因。本研究利用全长cDNA芯片分析了这些细胞色素P450基因在激素（水杨酸、茉莉酸、乙烯、脱落酸）、病原菌接种（芸苔链格孢菌、互隔交链孢菌）、百草枯、玫瑰红、紫外线（UV-C）、重金属（CuSO 4）、机械伤害、干旱、高盐和低温等处理下的表达模式。其中29个细胞色素P450基因的表达受不同处理的诱导。接种A. brassicicola和A. alternata作为生物胁迫分别提高了拟南芥中12个和5个基因的转录水平。此外，部分基因在非生物胁迫下也能表达。这表明非生物和生物应力之间的串扰。在全长cDNA序列的基础上研究了每个基因的启动子序列和顺式作用元件。大多数受非生物胁迫和生物胁迫诱导的细胞色素P450基因的启动子中都含有MYB和MYC的识别位点、ACGT核心序列、WRKY转录因子的TGA-box和W-box。已知这些顺式作用元件参与植物防御的调节。每个基因对多重压力的反应都受到严格的调控。
From Arabidopsis full-length cDNA libraries, we collected ca. 7000 (7K) independent full-length cDNAs to prepare a cDNA microarray. The 7K cDNA collection contains 49 cytochrome P450 genes. In this study, expression patterns of these cytochrome P450 genes were analyzed by a full-length cDNA microarray under various treatments, such as hormones (salicylic acid, jasmonic acid, ethylene, abscisic acid), pathogen-inoculation (Alternaria brassicicola, Alternaria alternata), paraquat, rose bengal, UV stress (UV-C), heavy metal stress (CuSO4), mechanical wounding, drought, high salinity and low temperature. Expression of 29 cytochrome P450 genes among them was induced by various treatments. Inoculation with A. brassicicola and A. alternata as biotic stresses increased transcript levels of 12 and 5 genes in Arabidopsis plants, respectively. In addition, some of the genes were also expressed by abiotic stresses. This suggests crosstalk between abiotic and biotic stresses. The promoter sequences and cis-acting elements of each gene were studied on the basis of full-length cDNA sequences. Most cytochrome P450 genes induced by both abiotic and biotic stresses contained the recognition sites of MYB and MYC, ACGT-core sequence, TGA-box and W-box for WRKY transcription factors in their promoters. These cis-acting elements are known to participate in the regulation of plant defense. The response of each gene to multiple stresses is strictly regulated.