Stimulatory effects of nitric oxide donors on gastric acid secretion in isolated mouse stomach
Stimulatory effects of nitric oxide donors on gastric acid secretion in isolated mouse stomach
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DOI：
10.1016/s0014-2999(01)00995-5
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发表时间：
2001-05-25
影响因子：
5
通讯作者：
Watanabe, K
中科院分区：
文献类型：
--
作者：
Hasebe, K;Horie, S;Watanabe, K
我们之前报道过内源性一氧化氮（NO）在胃酸分泌中的刺激作用。在本研究中，我们研究了 NO 供体对离体小鼠胃酸分泌的影响。硝普钠（100 μM-1 mM）以浓度依赖性方式抑制组胺（500 μM）诱导的胃酸分泌。此外，硝普钠消除了由氨甲酰甲胆碱（100μM）和迷走神经电刺激（10Hz）诱导的酸分泌。另一方面，75μM硝普钠不影响组胺诱导的酸分泌，其本身引起酸分泌增加。 75 ELM 硝普钠诱导的酸分泌被 10 muM 法莫替丁（一种组胺 H-2 受体拮抗剂）抑制。这些结果表明，高剂量的NO供体作用于胃壁细胞，导致抑制刺激的酸分泌，而低剂量的NO供体则促进含组胺细胞释放组胺，导致胃酸分泌增加。 (C) 2001 Elsevier Science B.V. 保留所有权利。
We previously reported on the stimulatory role of endogenous nitric oxide (NO) in gastric acid secretion. In the present study, we investigated the effects of NO donors on acid secretion in isolated mouse stomach. Nitroprusside (100 muM-1 mM) inhibited the gastric acid secretion induced by histamine (500 muM) in a concentration-dependent manner. In addition, nitroprusside abolished the acid secretion induced by bethanechol (100 muM) and by electrical stimulation (10 Hz) of the vagus nerve. On the other hand, nitroprusside, 75 muM, which did not affect the acid secretion induced by histamine, itself elicited an increase in acid secretion. The acid secretion induced by 75 ELM nitroprusside was inhibited by 10 muM famotidine, a histamine H-2 receptor antagonist. These results suggest that NO donors at high doses act on gastric parietal cells, resulting in inhibition of the stimulated acid secretion, and, at lower doses, facilitate histamine release from histamine-containing cells, leading to the increased acid secretion. (C) 2001 Elsevier Science B.V. All rights reserved.