An essential role of phosphatidylinositol 3-kinase in myogenic differentiation
An essential role of phosphatidylinositol 3-kinase in myogenic differentiation
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DOI：
10.1073/pnas.95.24.14179
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发表时间：
1998-11-24
影响因子：
11.1
通讯作者：
Vogt, PK
中科院分区：
文献类型：
--
作者：
Jiang, BH;Zheng, JZ;Vogt, PK
致癌基因p3k编码一种组成活性形式的磷脂酰肌醇3-激酶（PI 3-激酶；EC 2.7.1.137），在培养的鸡胚成肌细胞中强烈地增强了成肌分化。它增加了肌管的大小，并诱导肌肉特异性蛋白MyoD、肌球蛋白重链、肌酸激酶和desmin的水平升高。LY294002或PI 3激酶的显性阴性突变体抑制PI 3激酶活性会干扰成肌分化和肌肉特异性基因的诱导。因此，pi3激酶是肌肉特异性基因表达的上游介质，在肌肉形成过程中既是必要的，也是限速的。
The oncogene p3k, coding for a constitutively active form of phosphatidylinositol 3-kinase (PI 3-kinase; EC 2.7.1.137), strongly enhances myogenic differentiation in cultures of chicken-embryo myoblasts. It increases the size of the myotubes and induces elevated levels of the muscle-specific proteins MyoD, myosin heavy chain, creatine kinase, and desmin. Inhibition of PI 3-kinase activity with LY294002 or with dominant-negative mutants of PI 3-kinase interferes with myogenic differentiation and with the induction of muscle-specific genes. PI3-kinase is therefore an upstream mediator for the expression of the muscle-specific genes and is both necessary and rate-limiting for the process of myogenesis.