Impaired Maternal Behavior in Usp46 Mutant Mice: A Model for Trans-Generational Transmission of Maternal Care.
Impaired Maternal Behavior in Usp46 Mutant Mice: A Model for Trans-Generational Transmission of Maternal Care.
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DOI：
10.1371/journal.pone.0136016
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Ebihara S
中科院分区：
文献类型：
--
作者：
Umemura S;Imai S;Mimura A;Fujiwara M;Ebihara S
与C57BL/6J小鼠（B6小鼠）相比，Usp46突变小鼠（B6遗传背景的同源菌株；MT小鼠）断奶率低，母性行为差。基于这些观察，我们研究了MT和B6小鼠的交叉培养和内培养是如何塑造母性行为的。实验分为六组：由亲生母亲（B6- co）培养的B6小鼠；MT小鼠由其亲生母亲（MT- co）培养；不同B6母鼠培养的B6小鼠（B6- if）；由不同MT母培养的MT小鼠（MT- if）；MT母鼠培养B6小鼠（B6- cf）；B6母鼠培养的MT小鼠（MT- cf）。母鼠行为评估采用成年雌性后代的找回幼崽测试，并测量了4个参数，即在巢中哺育幼崽的时间、嗅探或舔舐幼崽的时间、饲养行为和找回幼崽的潜伏期。交叉饲养显著减少了B6-CF组哺乳和嗅舔幼犬的时间，增加了哺育次数，改善了MT-CF组母性行为的三个参数（哺育、哺育和潜伏期）。这些结果表明，母性照顾的水平会传递给它们的幼崽，如果产后给予足够的母性照顾，就可以塑造适当的母性行为，即使是基因脆弱的小鼠。然而，后代的基因型也可能影响成年后母亲行为的发展。因此，MT小鼠可能被证明是一种有用的跨代传递母亲照顾的模型，这些发现可能为人类虐待儿童的虐待行为机制提供见解。
Usp46 mutant mice (congenic strain on a B6 genetic background; MT mice) have a low weaning rate and display poor maternal behavior compared to C57BL/6J mice (B6 mice). Based on these observations, we examined how maternal behavior is shaped by cross-fostering and in-fostering MT and B6 mice. The experiments consisted of six groups: B6 mice fostered by their biological mother (B6-CO); MT mice fostered by their biological mother (MT-CO); B6 mice fostered by a different B6 mother (B6-IF); MT mice fostered by a different MT mother (MT-IF); B6 mice fostered by an MT mother (B6-CF); and MT mice fostered by a B6 mother (MT-CF). Maternal behavior was assessed using the pup-retrieval test in adult female offspring, and four parameters, time nursing pups in the nest, time sniffing or licking pups, rearing behavior, and latency to retrieve pups, were measured. Cross-fostering significantly reduced time spent nursing and sniffing/licking pup, and increased the number of instances of rearing in the B6-CF group, and improved three parameters of maternal behaviors (nursing, rearing and latency) in the MT-CF group. These results indicate that the level of maternal care is transmitted to their pups and proper maternal behaviors can be shaped if adequate postpartum maternal care is given, even in genetically vulnerable mice. However, the offspring’s genotype may also influence the development of maternal behaviors in adulthood. Thus, MT mice may prove useful as a model for trans-generational transmission of maternal care, and these findings may provide insight into the mechanisms of maltreating behaviors in human child abuse.