Serum biomarker of diabetic peripheral neuropathy indentified by differential proteomics.
Serum biomarker of diabetic peripheral neuropathy indentified by differential proteomics.
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DOI：
10.2741/3879
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发表时间：
2011-06
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影响因子：
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通讯作者：
Wei-Yu Tang;Yong-Quan Shi;J. Zou;Xiang-fang Chen;Jiao-yang Zheng;Shudong Zhao;Zhi-Min Liu
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文献类型：
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作者：
Wei-Yu Tang;Yong-Quan Shi;J. Zou;Xiang-fang Chen;Jiao-yang Zheng;Shudong Zhao;Zhi-Min Liu
至少有四分之一的糖尿病患者受到周围神经病变的影响。在本研究中，肽和蛋白质的MALDI-TOF-MS质谱是在39例糖尿病周围神经病变（DPN）、39例糖尿病（DM）和35例对照（CON）血清样本的WCX微球PROT珠分级分离后生成的。使用flexAnalysisTM和Clin-ProtTM生物信息学软件对光谱进行统计分析。进行所选标志物的鉴定，并对亲和珠纯化的血浆蛋白进行LTQ Orbitrap XL MS/MS分析，然后进行肽序列的Mascot鉴定。共鉴定出89个血清蛋白质差异表达峰。使用ClinProTool软件包分别选择CON与DM、CON与DPN、DM与DPN之间的17、10和4个最显著峰，并用于训练监督神经网络。所有集的准确率为100%。在该分析之后，6631-Da标记物被鉴定为载脂蛋白C-I前体的片段。所鉴定的肽可能具有临床实用性，可作为检测和分类DM和DPN的替代标记物。
At least one in four diabetic patients is affected by peripheral neuropathy. In this study, the MALDI-TOF-MS mass spectra of peptides and proteins were generated following WCX CLINPROT bead fractionation of 39 diabetic peripheral neuropathy (DPN), 39 diabetes mellitus (DM), and 35 control (CON) serum samples. The spectra were analyzed statistically using flexAnalysisTM and Clin-ProtTM bioinformatics software. Identification of the selected markers was performed and affinity bead-purified plasma protein was subjected to LTQ Orbitrap XL MS/MS analysis followed by Mascot identification of the peptide sequences. 89 differentially expressed peaks of serum proteins were identified. 17, 10 and 4 most significant peaks between CON vs. DM, CON vs. DPN, DM vs. DPN, respectively, were selected out using the ClinProTool software package and used to train a Supervised Neural Network. A veracity rate of 100% was obtained for all sets. Following this analysis, a 6631-Da marker was identified as a fragment of the Apolipoprotein C-I precursor. The peptides identified may have clinical utility as surrogate markers for detection and classification of DM and DPN.