Consensus numbering system for the rifampicin resistance-associated rpoB gene mutations in pathogenic mycobacteria
Consensus numbering system for the rifampicin resistance-associated rpoB gene mutations in pathogenic mycobacteria
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DOI：
10.1016/j.cmi.2016.09.006
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发表时间：
2017-03-01
影响因子：
14.2
通讯作者：
Cambau, E.
中科院分区：
文献类型：
--
作者：
Andre, E.;Goeminne, L.;Cambau, E.
rpo B基因编码RNA聚合酶B亚基，其是利福平的靶标，利福平是治疗结核病和其他分枝杆菌感染的基本药物。该基因存在于所有细菌中，但其长度和核苷酸序列在细菌物种之间存在差异，包括分枝杆菌。rpoB基因的突变改变了这种蛋白质的结构，并导致耐药性。为了描述耐药相关突变，自1993年以来，科学和医学界一直在使用基于大肠杆菌序列注释的编号系统。使用大肠大肠杆菌中描述分枝杆菌突变的参考文献导致误解，特别是随着全基因组测序的使用越来越多，这带来了基于结核分枝杆菌rpoB序列的替代编号系统。我们建议使用一个共识编号系统来报告耐药突变，该系统基于来自所调查物种的参考基因组（例如M.肺结核）。该手稿提供了必要的图表，使研究人员，微生物学家和临床医生能够轻松地将其他注释系统转换为一种通用语言。(C)2016作者（S）由Elsevier Ltd代表欧洲临床微生物学和传染病学会出版。
The rpoB gene codes for the RNA polymerase b subunit, which is the target of rifampicin, an essential drug in the treatment of tuberculosis and other mycobacterial infections. This gene is present in all bacteria, but its length and nucleotide sequence vary between bacterial species, including mycobacteria. Mutations in the rpoB gene alter the structure of this protein and cause drug resistance. To describe the resistance-associated mutations, the scientific and medical communities have been using, since 1993, a numbering system based on the Escherichia coli sequence annotation. Using E. coli reference for describing mutations in mycobacteria leads to misunderstandings, particularly with the increasing use of whole genome sequencing, which brought an alternative numbering system based on the Mycobacterium tuberculosis rpoB sequence. We propose using a consensus numbering system for the reporting of resistance mutations based on the reference genomes from the species interrogated (such as strain H37Rv for M. tuberculosis). This manuscript provides the necessary figures and tables allowing researchers, microbiologists and clinicians to easily convert other annotation systems into one common language. (C) 2016 The Author(s). Published by Elsevier Ltd on behalf of European Society of Clinical Microbiology and Infectious Diseases.