Genotyping of multidrug-resistant strains of Pseudomonas aeruginosa isolated from burn and wound infections by ERIC-PCR
Genotyping of multidrug-resistant strains of Pseudomonas aeruginosa isolated from burn and wound infections by ERIC-PCR
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DOI：
10.1590/s0102-865020160030000009
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发表时间：
2016-03-01
影响因子：
1.1
通讯作者：
Jenabi, Atefeh
中科院分区：
文献类型：
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作者：
Khosravi, Azar Dokht;Hoveizavi, Hajar;Jenabi, Atefeh
目的：应用ERIC-PCR法分析从伊朗阿瓦兹地区烧伤和创面感染中分离到的耐多药铜绿假单胞菌的遗传多样性。方法：从99株经药敏试验确定为耐多药的铜绿假单胞菌中，66株经ERIC-PCR法分析，其中包括53株烧伤感染分离株和13株对多种药物联合耐药性较高的创面感染分离株。烧伤感染分离株具有高度的异质性，包括16个分离株，分为8个类群和37个单克隆。第II、III、VI、VIII群的菌株具有100%的相似性。结论：铜绿假单胞菌具有高度的基因异质性，它们之间没有遗传相关性。烧伤分离株的耐药性极高，提示应采取有效的控制措施和适当的抗生素政策。
PURPOSE: To determine the genetic diversity of MDR P. aeruginosa strains isolated from burn and wound infections in Ahvaz, Iran, by ERIC-PCR.METHODS: From total 99 strains of P. aeruginosa defined as MDR by using drug susceptibility testing, 66 were subjected to ERIC-PCR analysis, comprises 53 strains isolated from burn infection, and 13 randomly selected strains from wound infection with higher resistance to combinations of more numbers of drugs.RESULTS: Eight clusters (I to VIII), and 50 single clones were generated for tested MDR isolates analyzed by ERIC-PCR. The high heterogeneity was observed among the isolates from burn infections including 16 isolates which were categorized in eight clusters and 37 single clones. The isolates in clusters II, III, VI, VIII showed 100% similarity.CONCLUSIONS: The high level of genotypic heterogeneity in P. aeruginosa strains demonstrated no genetic correlation between them. Extremely high drug resistance in isolates from burn, suggests that efficient control measures and proper antibiotic policy should be observed.