A histochemical study of sympathetic innervation and 5-hydroxytryptamine in the developing pineal body of the rat.
A histochemical study of sympathetic innervation and 5-hydroxytryptamine in the developing pineal body of the rat.
复制标题
大鼠松果体发育过程中交感神经支配和 5-羟色胺的组织化学研究。
DOI：
10.1016/0006-8993(68)90051-6
复制
发表时间：
1968
期刊：
影响因子：
2.9
通讯作者：
A. B. Machado
中科院分区：
文献类型：
--
作者：
C. Machado;L. Wragg;A. B. Machado
用法尔克和希拉普的荧光法研究了幼年大鼠松果体交感神经支配的发育和5-羟色胺（5-HT）的出现。首先出现的荧光是出生后2小时观察到的松果体囊中交感神经的绿色（儿茶酚胺）。24 h时，部分神经已穿入腺体。在第2天，大绿色神经被发现在外部和内部的松果体。在腺体内，可见曲张的终末从神经束中出现，在血管周围形成精致的网络，并在实质细胞中扩散。在第3天，在薄壁组织中出现明显的黄色荧光（5-HT），但它很弱且不均匀。所有神经都发出绿色荧光。在第6-7天，薄壁组织发出强黄色荧光，但存在仅发出轻微荧光的细胞簇。虽然大多数的松果体内神经是绿色，少数在这个年龄变得黄色。第10-11天，松果体实质荧光类似于成年腺体，松果体内神经呈明显黄色。静脉曲张终末的数量比成人腺体少。21日龄时，松果体与成年腺体相似。并对5-HT在脑实质细胞中的出现不依赖于其神经支配进行了讨论。
The development of sympathetic innervation and the appearance of 5-HT in the young rat pineal body was studied using the fluorescence method of Falck and Hillarp.There is no fluorescence in the pineal of the 19-day-old rat fetus. The first fluorescence to appear is the green (catecholamines) of the sympathetic nerves in the pineal capsule which was observed 2 h after birth. At 24 h some nerves had penetrated the gland. At day 2, large green nerves were found both outside and inside the pineal body. Inside the gland, varicose terminals were seen emerging from the nerve bundles, forming a delicate network around vessels and spreading out among parenchymal cells. At day 3 a distinctly yellow fluorescence (5-HT) appeared in the parenchyma but it was weak and uneven. All the nerves fluoresced green. At day 6–7 the parenchyma fluoresced strong yellow but there were clusters of cells only slightly fluorescent. Although most of the intrapineal nerves were green, a few were becoming yellowish at this age. At day 10–11 the yellow parenchymal fluorescence resembled that of the adult gland and the intrapineal nerves were distinctly yellow. The number of varicose terminals was smaller than in the adult gland. At 21 days the pineal gland appears similar to the adult gland. That the appearance of 5-HT in the parenchymal cells does not depend on its innervation is discussed.