GLUCOSE ENZYME ELECTRODE WITH EXTENDED LINEARITY - APPLICATION TO UNDILUTED BLOOD MEASUREMENTS
GLUCOSE ENZYME ELECTRODE WITH EXTENDED LINEARITY - APPLICATION TO UNDILUTED BLOOD MEASUREMENTS
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DOI：
10.1016/0003-2670(86)80074-5
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发表时间：
1986-05-31
影响因子：
6.2
通讯作者：
VADGAMA, PM
中科院分区：
文献类型：
--
作者：
MULLEN, WH;KEEDY, FH;VADGAMA, PM
本发明描述了一种酶电极，用于测定未搅拌、未稀释全血中的葡萄糖。该系统包括h2o2检测电极，在其上放置含有葡萄糖氧化酶的膜层压板。外膜采用甲基三氯硅烷预处理。电极响应与葡萄糖浓度呈线性关系，最高可达50 mmol l−1葡萄糖，对溶解氧浓度的依赖性较低，响应时间为30-90 s。全血糖测量值与常规分光光度法相关良好。
An enzyme electrodes is described for glucose determination in unstirred, undiluted whole blood. The system comprises an H2O2-detecting electrode upon which is placed a membrane laminate incorporating glucose oxidase. The external membrane was pretreated with methyltrichlorosilane. The electrode response was linearly dependent on glucose concentration up to 50 mmol l−1glucose, it had a decreased dependence on dissolved oxygen concentrations and gave response times of 30–90 s. Whole blood glucose measurements correlated well with a routine spectrophotometric method.