Imaging of mRNA in Live Cells Using Nucleic Acid-Templated Reduction of Azidorhodamine Probes
Imaging of mRNA in Live Cells Using Nucleic Acid-Templated Reduction of Azidorhodamine Probes
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DOI：
10.1021/ja809656k
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发表时间：
2009-05-13
影响因子：
15
通讯作者：
Winssinger, Nicolas
中科院分区：
文献类型：
--
作者：
Pianowski, Zbigniew;Gorska, Katarzyna;Winssinger, Nicolas
导致荧光产物的核酸模板反应是细胞核酸检测和成像的一种有吸引力的策略。在这里，我们报告了使用Staudinger反应来促进前荧光叠氮多罗丹明的还原。使用两个细胞渗透性的GPNA探针，一个标记有前荧光的叠氮多罗丹明，另一个标记有三烷基膦，使得能够在完整的细胞中检测到编码O-6-甲基鸟嘌呤-DNA甲基转移酶的mRNA。
Nucleic acid-templated reactions leading to a fluorescent product represent an attractive strategy for the detection and imaging of cellular nucleic acids. Herein we report the use of a Staudinger reaction to promote the reduction of profluorescent azidorhodamine. The use of two cell-permeable GPNA probes, one labeled with the profluorescent azidorhodamine and the other with trialkylphosphine, enabled the detection of the mRNA encoding O-6-methylguanine-DNA methyltransferase in intact cells.