Characterization of methicillin-resistant Staphylococcus aureus ST398 from cases of bovine mastitis
Characterization of methicillin-resistant Staphylococcus aureus ST398 from cases of bovine mastitis
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DOI：
10.1093/jac/dkq021
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发表时间：
2010-04-01
影响因子：
5.2
通讯作者：
Schwarz, Stefan
中科院分区：
文献类型：
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作者：
Fessler, Andrea;Scott, Carly;Schwarz, Stefan
对从德国17个奶牛场分离的25株临床型奶牛乳房炎MRSA ST 398菌株和2株来自奶牛场工作人员的MRSA ST 398菌株进行了亲缘关系、耐药性和毒力特性的研究，并采用ApaI PFGE、spa分型、SCCmec分型和dru分型方法进行了基因组亲缘关系的鉴定。采用微量肉汤稀释法测定耐药表型。利用基因芯片和特异性PCR技术检测出9种主要的ApaI PFGE图谱。鉴定出三种spa类型（t011、t034和t2576）和两种SCCmec类型（IV和V）。5种不同的药物类型，以dt 11 a为主。所有分离株均为Panton-Valentine杀白细胞素、肠毒素和剥脱毒素基因阴性。观察到10种耐药模式，其中11株（40.7%）仅对β-内酰胺类抗生素和四环素耐药。检测到几个抗性基因：blaZ（青霉素耐药性）;泰特（M）、泰特（K）和泰特（L）（四环素耐药）; β（A）、β（B）、β（C）和β（T）（大环内酯类/林可酰胺类/链阳性菌素B耐药）; aacA-aphD、aphA 3、aadD和spc（氨基糖苷类或氨基环醇耐药）（苯尼考抗性）; dfrK（甲氧苄啶抗性）;和vga（A）和vga（C）（截短侧耳素/林可酰胺/链阳性菌素A抗性）。这两个人的分离物是无法区分的基因型和表型特征的乳房炎分离物的同一farm.As先前所述的ST 398从猪，分离物的这种序列类型的牛乳房炎的情况下，也表现出高度的变异性时，ApaI PFGE配置文件和其他基因型和表型特征进行了比较。一个统一的毒力基因模式似乎是保守的ST 398菌株从两个动物物种。
Twenty-five MRSA ST398 isolates from cases of bovine clinical mastitis and two isolates from farm personnel collected from 17 dairy farms in Germany were investigated for genetic relatedness, antimicrobial resistance and virulence properties.Genomic relationships were determined by ApaI PFGE, spa typing, SCCmec typing and dru typing. Antimicrobial resistance phenotypes were determined by broth microdilution. Resistance and virulence genes were detected via a diagnostic DNA microarray and specific PCRs.Nine major ApaI PFGE patterns were detected. Three spa types (t011, t034 and t2576) and two SCCmec types (IV and V) were identified. Five different dru types were seen with dt11a being predominant. All isolates were negative for Panton-Valentine leucocidin, enterotoxin and exfoliative toxin genes. Ten resistance patterns were observed with 11 (40.7%) isolates being resistant to only beta-lactam antibiotics and tetracyclines. Several resistance genes were detected: blaZ (penicillin resistance); tet(M), tet(K) and tet(L) (tetracycline resistance); erm(A), erm(B), erm(C) and erm(T) (macrolide/lincosamide/streptogramin B resistance); aacA-aphD, aphA3, aadD and spc (aminoglycoside or aminocyclitol resistance); fexA (phenicol resistance); dfrK (trimethoprim resistance); and vga(A) and vga(C) (pleuromutilin/lincosamide/streptogramin A resistance). The two human isolates were indistinguishable in their genotypic and phenotypic characteristics from the mastitis isolates of the same farm.As previously described for ST398 from swine, isolates of this sequence type from cases of bovine mastitis also demonstrated a high degree of variability when ApaI PFGE profiles and other genotypic and phenotypic characteristics were compared. A uniform virulence gene pattern appeared to be conserved between ST398 isolates from both animal species.