POSITIVE SELECTION FOR MALE-STERILE MUTANTS OF ARABIDOPSIS LACKING ADENINE PHOSPHORIBOSYL TRANSFERASE-ACTIVITY
POSITIVE SELECTION FOR MALE-STERILE MUTANTS OF ARABIDOPSIS LACKING ADENINE PHOSPHORIBOSYL TRANSFERASE-ACTIVITY
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DOI：
10.1104/pp.86.4.1150
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发表时间：
1988-04-01
期刊：
影响因子：
7.4
通讯作者：
SOMERVILLE, C
中科院分区：
文献类型：
--
作者：
MOFFATT, B;SOMERVILLE, C
通过在含有0.1毫摩尔2，6-二氨基嘌呤的培养基上选择种子萌发来分离腺嘌呤磷酸核糖转移酶活性缺陷的拟南芥突变体。在每个突变体中，二氨基嘌呤抗性是由于在指定的apt位点处的隐性核突变。突变体生长比野生型慢，并且由于花粉母细胞减数分裂后花粉发育的败育而雄性不育。突变体的可靠性和容易性，可以选择应该提供新的机会，调查嘌呤代谢，花粉发育，遗传问题，需要的能力，选择功能丧失突变。
Three mutants of Arabidopsis thaliana deficient in adenine phosphoribosyl transferase activity were isolated by selecting for germination of seeds on a medium containing 0.1 millimolar 2,6-diaminopurine. In each of the mutants, diaminopurine resistance was due to a recessive nuclear mutation at a locus designated apt. The mutants grow more slowly than wild type, and are male sterile due to abortion of pollen development after the meiotic divisions of the pollen mother cells. The reliability and ease with which the mutants can be selected should afford novel opportunities to investigate purine metabolism, pollen development, and genetic problems which require the ability to select for loss-of-function mutations.