PhCESA3 silencing inhibits elongation and stimulates radial expansion in petunia.
PhCESA3 silencing inhibits elongation and stimulates radial expansion in petunia.
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DOI：
10.1038/srep41471
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发表时间：
2017-02-02
影响因子：
4.6
通讯作者：
Yu Y
中科院分区：
文献类型：
--
作者：
Yang W;Cai Y;Hu L;Wei Q;Chen G;Bai M;Wu H;Liu J;Yu Y
纤维素合成酶催化亚基（CESAs）在植物生长发育和抗病性中起着重要作用。先前的研究表明拟南芥CESA 3在植物生长中起重要作用。然而，关于CESA 3在A. thaliana.为了更好地理解CESA 3，分离矮牵牛（Petunia hybrida）PhCESA 3基因，并在广泛的植物中分析PhCESA 3在植物生长中的作用。PhCESA 3 mRNA在检查的组织中以不同的水平存在。VIGS介导的PhCESA3基因沉默导致植株高度矮化，这与A. thaliana rsw1突变体（AtCESA 1的温度敏感等位基因）、A. thaliana cev 1突变体（AtCESA 3轻度突变体）和反义AtCESA 3系。然而，PhCESA 3沉默导致茎、花梗、花丝、花柱和表皮毛肿胀，以及叶片和花冠增厚，这在A. thaliana cev 1突变体、rsw1突变体和反义AtCESA 3系。进一步的显微照片显示，PhCESA3沉默减少了细胞的长度并增加了细胞的宽度，表明PhCESA3沉默抑制了矮牵牛的伸长并刺激了矮牵牛的径向扩张。
Cellulose synthase catalytic subunits (CESAs) play important roles in plant growth, development and disease resistance. Previous studies have shown an essential role of Arabidopsis thaliana CESA3 in plant growth. However, little is known about the role of CESA3 in species other than A. thaliana. To gain a better understanding of CESA3, the petunia (Petunia hybrida) PhCESA3 gene was isolated, and the role of PhCESA3 in plant growth was analyzed in a wide range of plants. PhCESA3 mRNA was present at varying levels in tissues examined. VIGS-mediated PhCESA3 silencing resulted in dwarfing of plant height, which was consistent with the phenotype of the A. thaliana rsw1 mutant (a temperature-sensitive allele of AtCESA1), the A. thaliana cev1 mutant (the AtCESA3 mild mutant), and the antisense AtCESA3 line. However, PhCESA3 silencing led to swollen stems, pedicels, filaments, styles and epidermal hairs as well as thickened leaves and corollas, which were not observed in the A. thaliana cev1 mutant, the rsw1 mutant and the antisense AtCESA3 line. Further micrographs showed that PhCESA3 silencing reduced the length and increased the width of cells, suggesting that PhCESA3 silencing inhibits elongation and stimulates radial expansion in petunia.