CARDIOPROTECTIVE EFFECTS OF ETHANOLIC LEAF EXTRACT OF IPOMOEA BATATAS ON DOXORUBICIN INDUCED CARDIOTOXICITY IN RATS
CARDIOPROTECTIVE EFFECTS OF ETHANOLIC LEAF EXTRACT OF IPOMOEA BATATAS ON DOXORUBICIN INDUCED CARDIOTOXICITY IN RATS
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红薯乙醇叶提取物对阿霉素所致大鼠心脏毒性的心脏保护作用
DOI：
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
KP Shivalingegowda
中科院分区：
文献类型：
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作者：
B. Somashekar;S. Rai;KP Shivalingegowda
目的：本研究旨在通过检测白化病Wistar大鼠心肌酶、非酶抗氧化水平、血清酶水平、组织病理学检查和心电图改变，探讨番薯叶乙醇提取物（EEIB）对阿霉素（DOX）所致心脏毒性的保护作用。研究方法：分别以EEIB（300、600、900 mg/kg体重，n=6）和维生素C（50 mg/kg）灌胃10 d，第7天给予DOX 15 mg/kg。第11天，用麻醉乙醚麻醉大鼠，进行心电图、心脏组织生化及组织学观察。结果如下：当与DOX相比时，在提取物（600、900 mg/kg）和抗坏血酸预处理的大鼠中，心脏标志物如肌酸酐激酶（CK-MB）、CK、乳酸脱氢酶和脂质过氧化物的水平显著降低，具有显著性p<0.001。在心脏中，当与DOX相比时，在提取物（600、900 mg/kg）和抗坏血酸预处理的大鼠中，超氧化物歧化酶、过氧化氢酶、组织蛋白和谷胱甘肽水平显著增加，具有显著性p<0.001。心脏重量和心电图改变逐渐恢复的提取物以剂量依赖性的方式。浸提剂量为300 mg/kg时，上述参数无显著恢复。结论：研究表明，提取物和维生素C具有剂量依赖性的潜力，以防止由DOX诱导的心脏氧化损伤/自由基损伤。关键词：阿霉素，维生素C，甘薯，血清酶，抗氧化酶。
Objective: This study was aimed to investigate the protective effects of ethanolic leaf extract of Ipomoea batatas (EEIB) on doxorubicin (DOX) induced cardiotoxicity using Albino Wistar rats by monitoring the enzymatic, non-enzymatic antioxidant levels, serum enzyme levels, microscopical examinations and the study in electrocardiography (ECG) alterations. Methods: The rats were treated with EEIB (300, 600, 900 mg/kg body weight, n=6) and vitamin C (50 mg/kg) orally for 10 days and the rats were also treated with DOX 15 mg/kg on 7th day. On the 11th day, the rats were treated with anaesthetized with anesthetic ether, ECG and the levels of biochemical and histological observations of the heart tissues were performed. Results: The levels of cardiac markers like creatinine kinase (CK-MB), CK, lactate dehydrogenase and lipid peroxides were significantly decreased in the extract (600, 900 mg/kg) and ascorbic acid pretreated rats when compared to DOX with significance p<0.001. In heart superoxide dismutase, catalase, tissue protein and glutathione levels were significantly increased in the extract (600, 900 mg/kg) and ascorbic acid pretreated rats when compared to DOX with significance of p<0.001. The heart weight and electrographic alterations were gradually recovered by the extract in a dosedependent manner. There was no significant recovery of the above-mentioned parameters with an extract dose of 300 mg/kg. Conclusion: The study revealed that the extract and vitamin C have a dose-dependent potential to prevent oxidative damage/free radical damage induced by DOX in heart. Keywords: Doxorubicin, Vitamin C, Ipomoea batatas, Serum enzymes, Antioxidant enzymes.