Trichostatin A induces myocardial differentiation of monkey ES cells
Trichostatin A induces myocardial differentiation of monkey ES cells
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DOI：
10.1016/j.bbrc.2007.02.151
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发表时间：
2007-05-04
影响因子：
3.1
通讯作者：
Kita, Toru
中科院分区：
文献类型：
--
作者：
Hosseinkhani, Mohsen;Hasegawa, Koji;Kita, Toru
人胚胎干细胞系是终末期心力衰竭患者心肌细胞移植的可能来源之一。然而，在将ES细胞应用于人类心力衰竭治疗之前，关键是要验证其在大型动物如灵长类动物中的临床应用。食蟹猴ES细胞具有与人ES细胞相似的特性，可用于灵长类动物研究。我们证明，24小时的刺激组蛋白去乙酰化酶抑制剂，抑制素A（TSA）促进心肌分化的猴胚胎干细胞与胚胎体接种在明胶涂层的盘子。TSA诱导的组蛋白3/4乙酰化和p300表达，p300是一种内在的组蛋白乙酰转移酶。因此，这种诱导以及组蛋白脱乙酰酶的抑制可能参与TSA诱导的食蟹猴ES细胞分化为心肌细胞。(c)2007年爱思唯尔公司All rights reserved.
Human embryonic stem (ES) cell lines are one of the possible sources of cardiac myocytes to be transplanted in patients with end-staged heart failure. However, prior to the application of human of ES cells for heart failure therapy, it is critical to validate their clinical use in large animals such as primates. Cynomolgus monkey ES cells have similar properties to human ES cells and can be used for primate studies. We demonstrate that 24-h stimulation by a histone deacetylase inhibitor, trichostatin A (TSA) facilitated myocardial differentiation of monkey ES cells with embryonic bodies that were seeded on gelatin-coated dishes. TSA-induced acetylating of histone-3/4 and expression of p300, one of the intrinsic histone acetyltransferases. Thus, such induction as well as inhibition of histone deacetylase may be involved in TSA-induced differentiation of cynomolgus monkey ES cells into cardiomyocytes. (c) 2007 Elsevier Inc. All rights reserved.