Community structural differences shape microbial responses to high molecular weight organic matter
Community structural differences shape microbial responses to high molecular weight organic matter
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DOI：
10.1111/1462-2920.14485
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发表时间：
2019-02-01
影响因子：
5.1
通讯作者：
Arnosti, Carol
中科院分区：
文献类型：
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作者：
Balmonte, John Paul;Buckley, Andrew;Arnosti, Carol
微生物群落结构的差异在多大程度上导致有机物（OM）降解的变化还不清楚。在这里，我们测试的假设，不同的海洋微生物群落从北大西洋表面和底部沃茨将表现出不同的组成序列和功能的转变，在同一池的复杂的高分子量（HMW-OM）。我们还假设微生物群落在暴露于HMW-OM后会产生更广谱的酶，这表明降解这些化合物的潜力比初始酶活性所反映的更大。我们的研究结果表明，在修正后的围隔群落演替是一致的细胞生长，增加细菌产量，最值得注意的是，酶活性的实质性变化。在所有修正的围隔中，密切相关的类群，最初罕见的时间框架，在此期间，一个更广泛的活性酶被检测到的初始时间点相比，占主导地位，表明不同的社区之间的类似的反应。然而，在整个社区水平上的继承，以及酶活性的速率，光谱和进展，揭示了强大的不同社区之间的差异，从离散的水团。这些结果强调了稀有细菌类群在海洋碳循环中的关键作用以及细菌群落结构对HMW-OM降解的重要性。
The extent to which differences in microbial community structure result in variations in organic matter (OM) degradation is not well understood. Here, we tested the hypothesis that distinct marine microbial communities from North Atlantic surface and bottom waters would exhibit varying compositional succession and functional shifts in response to the same pool of complex high molecular weight (HMW-OM). We also hypothesized that microbial communities would produce a broader spectrum of enzymes upon exposure to HMW-OM, indicating a greater potential to degrade these compounds than reflected by initial enzymatic activities. Our results show that community succession in amended mesocosms was congruent with cell growth, increased bacterial production and most notably, with substantial shifts in enzymatic activities. In all amended mesocosms, closely related taxa that were initially rare became dominant at time frames during which a broader spectrum of active enzymes were detected compared to initial timepoints, indicating a similar response among different communities. However, succession on the whole-community level, and the rates, spectra and progression of enzymatic activities, reveal robust differences among distinct communities from discrete water masses. These results underscore the crucial role of rare bacterial taxa in ocean carbon cycling and the importance of bacterial community structure for HMW-OM degradation.