Toll-like receptors (TLR) 2 and 4 expression of keratinocytes from patients with localized and disseminated dermatophytosis.
Toll-like receptors (TLR) 2 and 4 expression of keratinocytes from patients with localized and disseminated dermatophytosis.
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DOI：
10.1590/s0036-46652015000100008
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发表时间：
2015-01
影响因子：
1.9
通讯作者：
Criado PR
中科院分区：
文献类型：
--
作者：
Oliveira CB;Vasconcellos C;Sakai-Valente NY;Sotto MN;Luiz FG;Belda Júnior W;Sousa Mda G;Benard G;Criado PR
先天免疫反应在皮肤癣菌病中的作用研究较少。本研究旨在明确Toll样受体（TLR）2和TLR 4在T.红色。本研究中使用了15例新诊断的患者，8例LD患者和7例DD患者，定义为至少累及3个身体部位。对照组包括来自接受整形手术的健康个体的20个皮肤样本。通过免疫组织化学定量皮肤病变中的TLR 2和TLR 4。与对照组相比，LD和DD患者的上下表皮中TLR 4的表达减少;所有三组的上下表皮中TLR 2的表达均被保留。由于TLR 4信号传导诱导炎性细胞因子的产生和嗜中性粒细胞募集，其表达减少可能导致感染缺乏消退和随后的皮肤真菌病的慢性性质。由于TLR 2表达起到限制炎症过程并保留表皮结构的作用，因此其保留的表达也可能有助于皮肤癣菌感染特征性的持续感染和有限炎症。
There are few studies on the role of innate immune response in dermatophytosis. An investigation was conducted to define the involvement of Toll-Like Receptors (TLRs) 2 and 4 in localized (LD) and disseminated (DD) dermatophytosis due to T. rubrum. Fifteen newly diagnosed patients, eight patients with LD and seven with DD, defined by involvement of at least three body segments were used in this study. Controls comprised twenty skin samples from healthy individuals undergoing plastic surgery. TLR2 and TLR4 were quantified in skin lesions by immunohistochemistry. A reduced expression of TLR4 in the lower and upper epidermis of both LD and DD patients was found compared to controls; TLR2 expression was preserved in the upper and lower epidermis of all three groups. As TLR4 signaling induces the production of inflammatory cytokines and neutrophils recruitment, its reduced expression likely contributed to the lack of resolution of the infection and the consequent chronic nature of the dermatophytosis. As TLR2 expression acts to limit the inflammatory process and preserves the epidermal structure, its preserved expression may also contribute to the persistent infection and limited inflammation that are characteristic of dermatophytic infections.