Spiking Characteristics of Network-Mediated Responses Arising in Direction-Selective Ganglion Cells of Rabbit and Mouse Retinas to Electric Stimulation for Retinal Prostheses.
Spiking Characteristics of Network-Mediated Responses Arising in Direction-Selective Ganglion Cells of Rabbit and Mouse Retinas to Electric Stimulation for Retinal Prostheses.
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DOI：
10.1109/tnsre.2021.3128878
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Im M
中科院分区：
文献类型：
--
作者：
Otgondemberel Y;Roh H;Fried SI;Im M
为了恢复因视网膜外部变性而失明的人的视力，人们已经开发出了许多视网膜假体。然而，这些植入物的性能仍然受到一些因素的阻碍，包括对各种类型的视网膜神经节细胞(RGC)产生的电诱发反应缺乏全面的了解。在本研究中，我们首次在兔和小鼠视网膜上研究了开-关方向选择(DS)视网膜节细胞的电诱发网络反应(以下简称电反应)。有趣的是，这两个物种都显示出电反应的尖峰计数与方向选择指数(DSI)之间的强烈负相关，这表明电刺激激活了抑制零方向反应的突触前神经元，从而抑制了光反应中的高方向调节。这两个物种的DS细胞表现出几个不同之处，包括不同的爆发数。此外，与小鼠相比，兔的DS视网膜节细胞的棘波模式更具异质性。兔DS细胞的电反应大小与择优运动的开、关光反应大小分别呈正相关和负相关。但在这两个比较中，小鼠的DS细胞显示出正相关。我们的Fano因子(FF)和穗时间平铺系数(STTC)分析表明，在两个物种的后期电反应中，重复序列上的穗一致性降低。此外，DS RGCs的反应一致性低于非DS RGCs。我们的结果表明，依赖于物种的视网膜回路可能会导致不同的电反应特征，因此，适当的动物模型可能在假体研究中至关重要。
To restore the sight of individuals blinded by outer retinal degeneration, numerous retinal prostheses have been developed. However, the performance of those implants is still hampered by some factors including the lack of comprehensive understanding of the electrically-evoked responses arising in various retinal ganglion cell (RGC) types. In this study, we characterized the electrically-evoked network-mediated responses (hereafter referred to as electric responses) of ON-OFF direction-selective (DS) RGCs in rabbit and mouse retinas for the first time. Interestingly, both species in common demonstrated strong negative correlations between spike counts of electric responses and direction selective indices (DSIs), suggesting electric stimulation activates inhibitory presynaptic neurons that suppress null direction responses for high direction tuning in their light responses. The DS cells of the two species showed several differences including different numbers of bursts. Also, spiking patterns were more heterogeneous across DS RGCs of rabbits than those of mice. The electric response magnitudes of rabbit DS cells showed positive and negative correlations with ON and OFF light response magnitudes to preferred direction motion, respectively. But the mouse DS cells showed positive correlations in both comparisons. Our Fano Factor (FF) and spike time tiling coefficient (STTC) analyses revealed that spiking consistencies across repeats were reduced in late electric responses in both species. Moreover, the response consistencies of DS RGCs were lower than those of non-DS RGCs. Our results indicate the species-dependent retinal circuits may result in different electric response features and therefore suggest a proper animal model may be crucial in prosthetic researches.