Antimicrobial factors in the cervical mucus plug
Antimicrobial factors in the cervical mucus plug
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DOI：
10.1067/mob.2002.123034
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发表时间：
2002-07-01
影响因子：
9.8
通讯作者：
Ganz, T
中科院分区：
文献类型：
--
作者：
Hein, M;Valore, EV;Ganz, T
目的：宫颈粘液栓位于微生物丰富的阴道和正常无菌的子宫腔之间，这表明宿主具有防御功能，但相关数据很少。我们分析了宫颈粘液plugs.Study设计的组成和抗菌活性：宫颈粘液塞收集健康妇女在分娩。结果：完整宫颈粘液栓及其水提物对需氧菌均有抗菌活性，其抗菌活性大小顺序为：B组链球菌>大肠杆菌>白色念珠菌。提取物的半定量Western印迹显示，分泌性白细胞蛋白酶抑制剂，溶菌酶，乳铁蛋白，和中性粒细胞防御素存在的浓度，是足够的抗菌活性。结论：宫颈粘液栓不仅是一个机械，但也是一个化学屏障，从阴道上升的感染。
OBJECTIVE: The cervical mucus plug is positioned between the microbe-rich vagina and the normally sterile uterine cavity, which suggests a host defense function, but few relevant data are available. We analyzed the composition and antimicrobial activity of cervical mucus plugs.STUDY DESIGN: Cervical mucus plugs were collected from healthy women at delivery. Groups of plugs were randomly selected for electrolyte analysis, antimicrobial activity assays against group B Streptococcus, Escherichia coli, Candida albicans, and assays of known antimicrobial polypeptides.RESULTS: Both intact cervical mucus plugs and their aqueous extracts exhibited antimicrobial activity against aerobic microbes, in the order of potency: group B Streptococcus > E coli > C albicans. Semiquantitative Western blotting of extracts showed that secretory leukoprotease inhibitor, lysozyme, lactoferrin, and neutrophil defensins were present at concentrations that were sufficient for antimicrobial activity.CONCLUSION: The cervical mucus plug is not only a mechanical but also a chemical barrier to infection that ascends from the vagina.