Facile detection of tumor-derived exosomes using magnetic nanobeads and SERS nanoprobes
Facile detection of tumor-derived exosomes using magnetic nanobeads and SERS nanoprobes
复制标题
使用磁性纳米珠和 SERS 纳米探针轻松检测肿瘤来源的外泌体
DOI：
10.1039/c6ay00406g
复制
发表时间：
2016-01-01
影响因子：
3.1
通讯作者：
Cui, Yiping
中科院分区：
文献类型：
--
作者：
Zong, Shenfei;Wang, Le;Cui, Yiping
外泌体在细胞间通讯中起重要作用。在这里，我们提出了一种基于表面增强拉曼散射（Sers）的检测方法，使用Sers纳米探针和磁性纳米珠的肿瘤来源的外泌体。Sers纳米探针具有核-壳结构，以金核-银壳纳米棒（Au@Ag NRs）为Sers活性核，拉曼分子为Sers报告分子，二氧化硅层为保护壳。Sers纳米探针的最外表面装饰有外来体特异性抗体。每个磁性纳米珠是通过用二氧化硅壳包覆Fe 3 O 4纳米颗粒（NPs）并将特异性抗体附着到二氧化硅壳来制造的。在存在靶外泌体的情况下，磁性纳米珠和Sers纳米探针可以通过形成免疫复合物来捕获外泌体。免疫复合物（以及Sers纳米探针）可以用磁体沉淀，因此可以在沉淀物中检测Sers信号。由于不存在靶外泌体，不能形成免疫复合物，因此在沉淀物中将检测到非常弱的Sers信号。因此，最终磁性分离产物的Sers信号可用于检测外来体。在实验中，使用一种肿瘤细胞和一种正常细胞，我们证明了所提出的方法可以用于定性和定量检测肿瘤源性exosomes。
Exosomes play an important role in intercellular communications. Here, we present a surface-enhanced Raman scattering (SERS) based detection method for tumor-derived exosomes using SERS nanoprobes and magnetic nanobeads. The SERS nanoprobe has a core-shell structure, with gold core-silver shell nanorods (Au@Ag NRs) as the SERS active core, Raman molecules as the SERS reporter and a silica layer as the protecting shell. The outmost surface of the SERS nanoprobe is decorated with exosome-specific antibodies. Each magnetic nanobead is fabricated by coating Fe3O4 nanoparticles (NPs) with a silica shell and attaching specific antibodies to the silica shell. With target exosomes present, the magnetic nanobeads and SERS nanoprobes can capture the exosomes by forming a sandwich-type immunocomplex. The immunocomplex (as well as the SERS nanoprobes) can be precipitated with a magnet, and thus SERS signals can be detected in the precipitates. With no target exosomes present, no immunocomplex can be formed, and thus quite weak SERS signals will be detected in the precipitates. Hence, the SERS signal of the final magnetic separation product can be used to detect exosomes. In the experiment, using one kind of tumor cells and one kind of normal cells, we proved that the presented method can be used for both qualitative and quantitative detection of tumor-derived exosomes.