Combinatorial mixing of microfluidic streams
Combinatorial mixing of microfluidic streams
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DOI：
10.1039/b314962e
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发表时间：
2004-01-01
期刊：
影响因子：
6.1
通讯作者：
Folch, A
中科院分区：
文献类型：
--
作者：
Neils, C;Tyree, Z;Folch, A
我们已经设计了一种微流体混合器设计，其产生输入化合物的给定数量的稀释液的所有混合物组合。作为概念的证明，我们提出了一种装置，该装置产生两种染料溶液（蓝色和黄色）的四种滴定，并将蓝色滴定与黄色滴定组合混合，以在单独的出口微通道中递送十六种混合物组合。我们的设备具有四个不同的流量水平，由堆叠九个激光切割聚酯薄膜层压板。流体网络具有对称设计，其保证所有出口处的流速相同，其中偏差可归因于制造、组装或灌注过程中的缺陷。设计规则按比例放大的化合物和/或稀释的数量。混合方案在高通量组合测试应用中具有广泛的适用性，例如药物筛选、基于细胞的生化测定、芯片实验室设备和生物传感器。
We have devised a microfluidic mixer design that produces all the mixture combinations of a given number of dilutions of the input compounds. As proof of the concept, we present a device that generates four titrations of two dye solutions, blue and yellow, and combinatorially mixes the blue titrations with the yellow titrations to deliver the sixteen mixture combinations in separate outlet microchannels. Our device features four different flow levels made by stacking nine laser-cut Mylar laminates. The fluidic network has a symmetric design that guarantees that the flow rates are the same at all the outlets, with deviations attributable to imperfections in the fabrication, assembly, or perfusion processes. Design rules for scaling up the number of compounds and/or dilutions are presented. The mixing scheme has broad applicability in high-throughput combinatorial testing applications such as drug screening, cell-based biochemical assays, lab-on-a-chip devices, and biosensors.