A FLUORESCENCE PHOTOBLEACHING ASSAY OF GAP JUNCTION-MEDIATED COMMUNICATION BETWEEN HUMAN-CELLS
A FLUORESCENCE PHOTOBLEACHING ASSAY OF GAP JUNCTION-MEDIATED COMMUNICATION BETWEEN HUMAN-CELLS
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DOI：
10.1126/science.3961495
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发表时间：
1986-04-25
期刊：
影响因子：
56.9
通讯作者：
SCHINDLER, M
中科院分区：
文献类型：
--
作者：
WADE, MH;TROSKO, JE;SCHINDLER, M
间隙连接介导的相邻细胞之间的通信已经涉及细胞增殖和分化的调节。本报告描述了一种新的技术来测量细胞间的通信，间隙荧光再分布后的光漂白，这是基于扩散依赖返回的6-羧基荧光素介导的荧光在光漂白的细胞，是在与其他荧光标记的细胞接触。荧光恢复率被解释为穿过间隙连接的染料传输。对正常人成纤维细胞和人畸胎癌细胞的实验结果表明，该技术可以测量快速染料转移，并检测肿瘤促进剂12-O-十四酰基-佛波醇-13-乙酸酯和农药狄氏剂对（畸胎癌细胞之间）通讯的抑制。
Gap junction-mediated communication between contiguous cells has been implicated in the regulation of cell proliferation and differentiation. This report describes a new technique to measure cell-cell communication, gap fluorescence redistribution after photobleaching, which is based on the diffusion-dependent return of 6-carboxyfluorescein-mediated fluorescence in a photobleached cell that is in contact with other fluorescently labeled cells. Fluorescence recovery rates are interpreted as dye transport across gap junctions. Results of experiments on normal human fibroblasts and human teratocarcinoma cells show that this technique can measure rapid dye transfer and detect inhibition of communication (between teratocarcinoma cells) by the tumor promoters 12-O-tetradecanoyl-phorbol-13-acetate and the pesticide dieldrin.