The major route for chlorophyll synthesis includes [3,8-divinyl]-chlorophyllide a reduction in Arabidopsis thaliana
The major route for chlorophyll synthesis includes [3,8-divinyl]-chlorophyllide a reduction in Arabidopsis thaliana
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DOI：
10.1093/pcp/pcm153
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发表时间：
2007-12-01
影响因子：
4.9
通讯作者：
Tanaka, Ayumi
中科院分区：
文献类型：
--
作者：
Nagata, Nozomi;Tanaka, Ryouichi;Tanaka, Ayumi
在大多数关于Chl生物合成的综述中，Chl被描述为通过涉及[3，8：-二乙烯基]-原叶绿素a的还原的途径合成。然而，Chl中间体的二乙烯基形式转化为其单乙烯基形式的可能性仍然存在于其他酶促步骤中。为了确定叶绿素生物合成的实际途径，我们在体外研究了以前命名为[3，8-二乙烯基]-原叶绿素a 8-乙烯基还原酶（DVR）的底物特异性。此外，我们调查了体内黄化幼苗中各种叶绿素中间产物的积累。总的来说，这些研究表明，[3，8-二乙烯基]-叶绿素a是DVR的主要底物。
In most reviews on Chl biosynthesis, Chl is described as being synthesized via the route involving the reduction of [3,8:-divinyl]-protochlorophyllide a. However, the possibility remains that the conversion of the divinyl form of the Chl intermediate to its monovinyl form takes place at other enzymatic steps. To determine the actual route of Chl biosynthesis, we examined the substrate specificity of the formerly named [3,8-divinyl]-protochlorophyllide a 8-vinyl reductase (DVR) in vitro. In addition, we investigated the accumulation of various Chl intermediates in etiolated seedlings in vivo. Collectively, these studies indicate that [3,8-divinyl]-chlorophyllide a is the major substrate of DVR.