Regulation of complexin 1 and complexin 2 in the developing human prefrontal cortex
Regulation of complexin 1 and complexin 2 in the developing human prefrontal cortex
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DOI：
10.1002/syn.20492
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发表时间：
2008-04-01
期刊：
影响因子：
2.3
通讯作者：
Jarskog, L. Fredrik
中科院分区：
文献类型：
--
作者：
Salimi, Kayvon;Glantz, Leisa A.;Jarskog, L. Fredrik
复合蛋白1（CX 1）和复合蛋白2（CX 2）是调节神经递质释放的突触前蛋白，分别用作抑制性和兴奋性突触的标志物。本研究的目的是通过检测CX 1和CX 2在人死后组织中的表达来了解人前额叶皮层（PFC）抑制性和兴奋性突触的发育。采用Western印迹法检测42名无脑损伤的受试者死后背外侧前额叶皮层（DLPFC）中复合蛋白的相对水平。神经或精神疾病，年龄范围为18孕周至25岁。将样本事先分批分为胎儿、0-12个月、1-5岁、6-10岁、11- 1 - 5岁、16-20岁和21-25岁年龄组。方差分析显示，年龄组对CX 1和CX 2表达及CX 2/CX 1有显著影响。组CX 1水平在整个发育过程中逐渐增加，在胎儿组中最低，在年轻成人组中最高，而组CX 2水平在胎儿组和6-10岁组之间增加，然后达到平台。与这些不同的模式相一致，年龄组对CX 2/CX 1有显著影响，胎儿和婴儿组高于年轻成人组。此外，回归分析表明，CX 1和CX 2/CX 1与年龄呈线性关系，而CX 2更好地描述为具有曲线关系与年龄。这些数据表明，在人类DLPFC的突触成熟过程中，复合蛋白的表达增加，并且在该皮质区域的发育过程中，抑制性突触的影响相对于兴奋性突触的影响有所增加。
Complexin 1 (CX1) and complexin 2 (CX2) are presynaptic proteins that modulate neurotransmitter release and are used as markers of inhibitory and excitatory synapses, respectively. The aim of this study was to gain insight into the development of inhibitory and excitatory synapses in human prefrontal cortex (PFC) by examining the expression of CX1 and CX2 in postmortem tissues. Relative complexin protein levels were measured by Western blotting in postmortem dorsolateral prefrontal cortex (DLPFC) of 42 subjects without. neurological or psychiatric disease ranging in age from 18 gestational weeks to 25 years. Samples were batched a priori into fetal, 0-12 month, 1-5 years, 6-10 years, 11-15 years, 16-20 years, and 21-25 years age groups. CX1 and CX2 expression and CX2/CX1 demonstrated a significant effect of age group by ANOVA. Group CX1 level increased progressively across development and was lowest in the fetal group and highest in the young adult group, whereas group CX2 level increased between the fetal and the 6-10 years groups and then plateaued. Consistent with these divergent patterns, there was a significant effect of age group on CX2/CX1, which was higher in fetal and infant groups than in the young adult group. Furthermore, regression analysis demonstrated linear relationships of CX1 and CX2/CX1 with age, whereas CX2 was better described as having a curvilinear relationship with age. These data indicate that complexin expression increases during synaptic maturation in human DLPFC and that an increase in the influence of inhibitory synapses relative to that of excitatory synapses occurs during development in this cortical region.