SYT-SSX fusion proteins in synovial sarcomas: detection and characterization with new antibodies
SYT-SSX fusion proteins in synovial sarcomas: detection and characterization with new antibodies
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DOI：
10.1016/s0304-3835(99)00336-5
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发表时间：
2000-02-28
期刊：
影响因子：
9.7
通讯作者：
Ochi, T
中科院分区：
文献类型：
--
作者：
Hashimoto, N;Araki, N;Ochi, T
为了鉴定和表征滑膜肉瘤中的SYT-SSX融合蛋白，我们开发了针对SYT-SSX2蛋白的N-末端部分和C-末端部分的两种多克隆抗体。在用两种亚型SYT-SSX融合基因SYT-SSX1和SYT-SSX2转染的COS-7细胞上证明了特异性。两种抗体都识别转染的COS-7细胞裂解物免疫沉淀中的单一61 kDa蛋白质。这些抗体还用免疫沉淀法在人滑膜肉瘤细胞系（HS-SY 41）的裂解物中检测到61 kDa的天然蛋白，并且用蛋白质印迹分析在人滑膜肉瘤的提取物中检测到61 kDa的天然蛋白。使用人滑膜肉瘤组织的免疫组织化学研究表明，SYT-SSX融合蛋白定位于肿瘤细胞的细胞核中。这些抗体为研究SYT-SSX融合蛋白的表达提供了有用的方法。(C)2000爱思唯尔科学爱尔兰有限公司保留所有权利。
To identify and characterize the SYT-SSX fusion proteins in synovial sarcomas, we developed two polyclonal antibodies against the N-terminal part and for the C-terminal part of the SYT-SSX2 protein. Specificity was demonstrated on COS-7 cells transfected with two subtypes of SYT-SSX fusion genes, SYT-SSX1 and SYT-SSX2. Both antibodies recognized a single protein of 61 kDa in an immunoprecipitation of the transfected COS-7 cell lysates. These antibodies also detected the native protein of 61 kDa in the lysate of a human synovial sarcoma cell line (HS-SY41) with immunoprecipitation, and in extracts of human synovial sarcomas with western blot analysis. An immunohistochemical study, using human synovial sarcoma tissues, demonstrated that the SYT-SSX fusion proteins localized in the nucleus of the tumor cells. These antibodies provide a useful method for studying the expression of the SYT-SSX fusion proteins. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.