Complementary homeo protein gradients in developing limb buds.
Complementary homeo protein gradients in developing limb buds.
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DOI：
10.1101/gad.3.5.641
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发表时间：
1989-05
影响因子：
10.5
通讯作者：
G. Oliver;N. Sidell;W. Fiske;C. Heinzmann;T. Mohandas;Robert S. Sparkes;E. M. D. Robertis
中科院分区：
文献类型：
--
作者：
G. Oliver;N. Sidell;W. Fiske;C. Heinzmann;T. Mohandas;Robert S. Sparkes;E. M. D. Robertis
克隆了一个新的人类同源盒基因Hox-5.2，并定位于人类2号染色体。这个同源盒在序列上与Abdominal-B相关，Abdominal-B是一种果蝇同源异型基因，指定后节的身份。使用人Hox-5.2融合蛋白制备抗体探针，发现其可染色小鼠、鸡和非洲爪蟾胚胎的后部区域。出乎意料的是，当将Hox-5.2抗原的分布与X1 Hbox 1抗原的分布进行比较时，检测到非重叠和互斥的模式（例如，在发育的肢芽、肠和体节中）。表达Hox-5.2的区域不表达X1 Hbox 1蛋白，反之亦然。Hox-5.2抗原在发育中的前肢和后肢芽中被强烈检测到，在那里它在大部分间充质中形成核蛋白梯度。该梯度在远端和后部区域最大。Hox-5.2的表达在爪蟾肢体再生胚基中被激活，正如任何参与模式形成的基因所预期的那样。如前所述，可以在前肢中检测到X1 Hbox 1蛋白的梯度。后一种梯度与Hox-5.2的极性相反。也就是说，在前中胚层和近中胚层中最大。这两个相反的梯度（可能还有其他梯度）可能参与决定发育中肢芽的位置值。
A new human homeo box-containing gene designated Hox-5.2 was cloned and mapped to human chromosome 2. This homeo box is related in sequence to Abdominal-B, a Drosophila homeotic gene that specifies identity of posterior segments. An antibody probe was made using a human Hox-5.2 fusion protein and was found to stain posterior regions of mouse, chicken, and Xenopus embryos. Unexpectedly, when the distribution of Hox-5.2 antigen was compared with that of X1Hbox 1 antigen, a non-overlapping and mutually exclusive pattern was detected (e.g., in developing limb buds, intestine, and somites). Regions expressing Hox-5.2 do not express X1Hbox 1 protein, and vice versa. Hox-5.2 antigen is detected strongly in developing fore- and hindlimb buds, where it forms a gradient of nuclear protein throughout most of the mesenchyme. This gradient is maximal in distal and posterior regions. Hox-5.2 expression is activated in Xenopus limb regeneration blastemas, as expected for any gene involved in pattern formation. As described previously, a gradient of X1Hbox 1 protein can be detected in the forelimb. The latter gradient has the opposite polarity to that of Hox-5.2. i.e., maximal in anterior and proximal mesoderm. These two opposing gradients (and possibly others) could be involved in determining positional values in developing limb buds.