High throughput qPCR unveils shared antibiotic resistance genes in tropical wastewater and river water.
High throughput qPCR unveils shared antibiotic resistance genes in tropical wastewater and river water.
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高通量 qPCR 揭示了热带废水和河水中共有的抗生素抗性基因。
DOI：
10.1016/j.scitotenv.2023.167867
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Srathongneam T
中科院分区：
文献类型：
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作者：
Srathongneam T
抗生素耐药性上升带来的全球挑战以及“一个健康”方法的采用，导致在各种环境中优先监测抗生素耐药基因（ARG）。这就存在着一个信息空白，特别是在泰国，那里缺乏关于不同环境矩阵和不同季节的ARG流行率的数据。本研究旨在填补这一空白，通过高通量qPCR分析流入物（n= 12）和流出物废水（n = 12）和河水（n = 12）中的ARG流行率。该研究揭示了所有水样类型中大量且基本上均匀的ARG存在（87%相似性）。有趣的是，没有ARG是专为特定的水类型，这表明整个水生环境的阻力决定因素的广泛循环。整合子和移动的遗传元件组中的基因intI 1、tnpA和intI 3在废水和河水样品中均检测到较高的相对丰度，表明废水对河流的污染广泛存在。所有水类型中其他高流行率ARG包括qepA、aadA 2、merA、sul 1、qacF/H、sul 2、aadB和dereA。更令人担忧的是，一些ARG（例如，blaVIM、intI 3、mcr-1、mexB、qepA、vanA和vanB）在流出物和河水中的相对丰度高于流入物，这表明废水处理工程故障或不足，并暗示这是环境污染的可能机制。九个基因（即，blaCTX-M、blaVIM、emrD、ermX、intI 1、mphA、qepA、vanA和vanB）在旱季的河水样品中的相对丰度高于雨季，这表明废水处理方法的有效性和进入接收沃茨的污染模式存在季节性影响。这项研究强调了采取更有效措施减少抗生素耐药性在供水系统中传播的紧迫性。
The global challenge posed by rising antimicrobial resistance, and the adoption of a One Health approach, has led to the prioritisation of surveillance for antibiotic resistance genes (ARGs) in various environments. Herein lies an information gap, particularly in the context of Thailand, where there is scarce data on ARG prevalence across diverse environmental matrices and throughout different seasons. This study aimed to fill this void, analysing ARG prevalence by high-throughput qPCR in influent (n= 12) and effluent wastewater (n = 12) and river water (n = 12). The study reveals a substantial and largely uniform presence of ARGs across all water sample types (87 % similarity). Intriguingly, no ARGs were exclusive to specific water types, indicating an extensive circulation of resistance determinants across the aquatic environment. The genesintI1,tnpA, andintI3, part of the integrons and mobile genetic elements group, were detected in high relative abundance in both wastewater and river water samples, suggesting widespread pollution of rivers with wastewater. Additional high-prevalence ARGs across all water types includedqepA,aadA2,merA,sul1,qacF/H,sul2,aadB, andereA. More alarmingly, several ARGs (e.g.,blaVIM,intI3,mcr-1,mexB,qepA,vanA, andvanB) showed higher relative abundance in effluent and river water than in influents, which suggests malfunctioning or inadequate wastewater treatment works and implicates this as a possible mechanism for environmental contamination. Nine genes (i.e.,blaCTX-M,blaVIM,emrD,ermX,intI1,mphA,qepA,vanA, andvanB) were recovered in greater relative abundance during the dry season in river water samples as compared to the wet season, suggesting there are seasonal impacts on the efficacy of wastewater treatment practices and pollution patterns into receiving waters. This study highlights the urgency for more effective measures to reduce antibiotic resistance dissemination in water systems.