Minimal compression of ultrathin sections with use of an oscillating diamond knife
Minimal compression of ultrathin sections with use of an oscillating diamond knife
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DOI：
10.1046/j.1365-2818.2000.00638.x
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发表时间：
2000-01-01
影响因子：
2
通讯作者：
Gnaegi, H
中科院分区：
文献类型：
--
作者：
Studer, D;Gnaegi, H
为了最大限度地减少超薄切片中的压缩伪影，与行程方向一致，我们发明了一种振动金刚石刀。讨论了解释其功能的结果和理论考虑。在传统的超薄切片过程中，产生的压缩约为切片高度的 20-35%。这对于嵌入 Lowicryl HM20 和聚合物聚苯乙烯的样品切片来说是正确的，用 45 度金刚石刀切割并漂浮在水面上。振动刀几乎完全减少了这种压缩。它由安装有低压压电转换器（piezo）的金刚石刀组成，当压电转换器由交流电压源驱动时，该转换器会发生振荡。当与不振动刀的情况下制作的切片进行比较时，在显微照片中没有观察到额外的切割伪影。
With the aim to minimize compression artefacts in ultrathin sections, coincident with the stroke direction, we have invented an oscillating diamond knife. Results and theoretical considerations explaining its function are discussed. During conventional ultrathin sectioning the resultant compression is in the order of 20-35% of section height. This holds true for sections of samples embedded into Lowicryl HM20 and of the polymer polystyrene, cut with a 45 degrees diamond knife and floated on water. The oscillating knife reduces this compression almost completely. It consists of a diamond knife on which a low voltage piezoelectric translator (piezo) is mounted, which oscillates when the piezo is driven by an alternating voltage source. No additional cutting artefacts were observed in the micrographs when they were compared with sections produced without oscillating the knife.