Quantitation of bacterial infection and antibiotic effect in the cornea.
Quantitation of bacterial infection and antibiotic effect in the cornea.
复制标题
角膜中细菌感染和抗生素作用的定量。
DOI：
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发表时间：
1976
期刊：
影响因子：
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通讯作者：
H. Leibowitz
中科院分区：
文献类型：
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作者：
A. Kupferman;H. Leibowitz
我们报告了一种实验模型，可以对细菌性角膜炎进行客观定量。该模型允许在不同的体内生长时期后直接测量角膜中活生物体的数量。用于产生角膜感染的接种物的大小至关重要，并且实验生物体必须针对其在角膜中的生长特征进行标准化。终点是客观的，产生可用于统计分析的数字数据。研究结果具有高度可重复性，并且该系统足够灵敏，足以表明局部施用抗生素能够减少远交兔群体角膜中活生物体的数量。
We report an experimental model that allows objective quantitation of bacterial keratitis. The model permits direct measurement of the number of viable organisms in the cornea after varying periods of in vivo growth. The size of the inoculum used to produce the corneal infection is critical, and the experimental organism must be standardized for its growth characteristics in the cornea. The end point is an objective one, productive of numerical data that can be subjected to statistical analysis. The findings are highly reproducible and the system is sufficiently sensitive to indicate the ability of a topically administered antibiotic to reduce the number of viable organisms in the cornea of an outbred rabbit population.