Autoinduction of differentiation in WEHI-3B leukemia cells.
Autoinduction of differentiation in WEHI-3B leukemia cells.
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WEHI-3B 白血病细胞的自诱导分化。
DOI：
10.1002/ijc.2910300616
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发表时间：
1982
影响因子：
6.4
通讯作者：
Nicola,NA
中科院分区：
文献类型：
--
作者：
Metcalf,D;Nicola,NA
分化反应性小鼠粒单核细胞白血病细胞系 WEHI-3B D+ 的细胞在琼脂中形成集落，主要在巨噬细胞途径中表现出低频率的自发分化。与未分化集落相比，自发分化集落的克隆形成细胞含量减少，并且存活的克隆形成细胞倾向于自身形成分化集落，这两者都是粒细胞集落刺激因子 G-CSF 诱导的分化集落的特征。集落拥挤增加了集落自发分化的频率，并且 WEHI-3B D+集落细胞被证明可以释放能够诱导 WEHI-3B D+集落分化的物质。与来自未分化集落的细胞相比，来自自发分化的 D+集落的细胞对 G-CSF 的分化诱导没有过度反应，并且不会释放更多量的分化诱导物质。分化无反应的 WEHI-3B D- 系细胞产生的分化诱导物质的量与 D+ 细胞产生的分化诱导物质的量相似。显然，WEHI-3B D+集落中的自发分化似乎最有可能是由于集落形成细胞或其祖先在琼脂培养之前暴露于 WEHI-3B 来源的分化诱导因子，启动分化的遗传程序由暴露细胞的后代遗传。
Cells of the differentiation‐responsive mouse myelomonocytic leukemia cell line WEHI‐3B D+form colonies in agar exhibiting a low frequency of spontaneous differentiation mainly in the macrophage pathway. Compared with undifferentiated colonies, spontaneously differentiating colonies have a reduced content of clonogenic cells and surviving clonogenic cells tend themselves to form differentiating colonies, both being characteristics of differentiated colonies induced by the regulator, granulocyte colony‐stimulating factor, G‐CSF. Colony crowding increased the frequency of spontaneously differentiating colonies and WEHI‐3B D+colony cells were shown to release material able to induce differentiation in WEHI‐3B D+colonies. Cells from spontaneously differentiating D+colonies were not hyperresponsive to the induction of differentiation by G‐CSF and did not release larger amounts of differentiation‐inducing material than did cells from undifferentiated colonies. Cells of the differentiation‐unresponsive WEHI‐3B D−line produced similar amounts of differentiation‐inducing material to those produced by D+cells. Apparently spontaneous differentiation in WEHI‐3B D+colonies seems most likely to be due to exposure of the colony‐forming cell or its ancestors to a differentiation‐inducing factor of WEHI‐3B origin prior to culture in agar, the genetic program initiating differentiation being inherited by the progeny of the exposed cell.