Development of a Real-Time PCR Approach for the Specific Detection of Prunus mahaleb
Development of a Real-Time PCR Approach for the Specific Detection of Prunus mahaleb
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开发用于特异性检测马哈莱布的实时 PCR 方法
DOI：
10.4236/fns.2016.78071
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
G. Nixon
中科院分区：
文献类型：
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作者：
M. Burns;M. Walker;T. Wilkes;L. Hall;Kirstin Gray;G. Nixon
2015年，一些孜然香料产品被从国际市场上召回，因为怀疑存在杏仁，杏仁是一种已知的李属过敏原。然而，免疫分析结果无法提供李属物种存在的明确证据，因为它与李属内的其他物种具有显著的交叉反应。建立了一种新的实时荧光聚合酶链式反应方法，用于特异性检测杏仁DNA，该物种已知能够在杏仁免疫分析中引起假阳性。该方法是基于来自内部转录间隔区(ITS)区域的现有DNA序列信息开发的，并与李属中的代表性物种进行了测试，以确保没有交叉反应。结果显示，在之前的国际召回事件中，在一种孜然香料产品中检测到了马哈莱布DNA，仅用免疫分析方法无法明确识别这一点。这份简短的报告详细说明了应用该分析的初步结果，并将对参与成分痕量检测的分析实验室感兴趣，以支持相关的食品标签立法。
In 2015 a number of cumin spice products were withdrawn from the international market because of the suspected presence of almond, a known allergen from the Prunus genus. However, immunoassay results were unable to provide unequivocal evidence of the Prunus species present, because of significant cross-reactivity with other species within the Prunus genus. A novel real-time PCR assay was developed for the specific detection of Prunus mahaleb DNA, a species known to be capable of causing false positives in almond immunoassays. The assay was developed based on available DNA sequence information from the Internal Transcribed Spacer (ITS) region, and tested against representative species within the Prunus genus to ensure no cross-reactivity. Results showed that mahaleb DNA was detected in a cumin spice product subject to the earlier international recalls, which could not be unequivocally identified using immunoassay approaches alone. This short report details preliminary results from the application of this assay, and will be of interest to analytical laboratories involved in trace detection of ingredients in support of relevant food labelling legislation.