Application of an avalanche photodiode in a near-Geiger operation as a fluorescence detector for capillary electrophoresis
Application of an avalanche photodiode in a near-Geiger operation as a fluorescence detector for capillary electrophoresis
复制标题
DOI：
10.1016/0021-9673(96)00385-8
复制
发表时间：
1996-09-13
影响因子：
4.1
通讯作者：
Ogawa, T
中科院分区：
文献类型：
--
作者：
Kawazumi, H;Song, JM;Ogawa, T
采用半导体激光器（655nm）作为激发光源，雪崩光电二极管（APD）作为荧光检测器，研制了毛细管电泳激光诱导荧光检测系统。通过调整APD的偏置电压，可以在近盖革条件下使用，其对光电子的内部增益大大增加。在近盖革操作中使用APD进行亚纳秒时间分辨光子计数，由于其光电子脉冲形状明确，计数效率提高，提供了非常高的灵敏度。Oxazine 725的检出限为47amol，比光电倍增管的检出限提高了约100倍。
A laser-induced fluorescence detection system for capillary electrophoresis has been developed by use of a semiconductor laser (655 nm) as an excitation light source and an avalanche photodiode (APD) as a fluorescence detector. The APD may be used in a near-Geiger condition by adjusting its bias voltage, and its internal gain to photoelectrons increases substantially. Subnanosecond time-resolved photon counting using the APD in the near-Geiger operation has provided very high sensitivity due to its well-defined photoelectron pulse shape and an enhancement of the counting efficiency. The detection limit of Oxazine 725 was 47 amol, which was about 100 times better than that using a photomultiplier.