NET PROTON INFLUX INTO BONE DURING METABOLIC, BUT NOT RESPIRATORY, ACIDOSIS
NET PROTON INFLUX INTO BONE DURING METABOLIC, BUT NOT RESPIRATORY, ACIDOSIS
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DOI：
10.1152/ajprenal.1988.254.3.f306
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发表时间：
1988-03-01
影响因子：
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通讯作者：
BUSHINSKY, DA
中科院分区：
文献类型：
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作者：
BUSHINSKY, DA
在急性代谢性酸中毒期间，质子净流入骨中，降低了升高的质子浓度。在急性呼吸性酸中毒期间是否有质子流入骨内尚不清楚。为了确定呼吸性酸中毒对相对于骨的净质子通量（JH）的影响，我们比较了新生小鼠颅骨在由PCO 2增加（呼吸性酸中毒）酸化的培养基中孵育3小时的JH与在由碳酸氢盐浓度降低（代谢性酸中毒）酸化至相同程度的培养基中孵育的颅骨的JH。呼吸性酸中毒的初始培养基pH与代谢性酸中毒的初始培养基pH没有差异（7.108 ± 0.001）。0.005对7.091 ±。0.007，P = NS）。在呼吸性酸中毒期间，相对于培养基，没有来自骨的JH（JH = 236 . ±. 93 neq. cntdot. bone-1.cntdot. 3 h-1，P = NS vs. 0）;然而，在代谢性酸中毒期间，存在从培养基流入骨的净质子（JH = -703 . ±. 108，P < 0.05 vs 0，P < 0.001 vs呼吸性酸中毒）。呼吸性酸中毒时骨钙流出量少于代谢性酸中毒时（JCa = 68 ± 0.01）。6 nmol·cm·dot. bone-1.cntdot. 3 h-1 vs. 100 .+-。9（P < 0.001）。在急性代谢性酸中毒时，有质子净流入体外骨中，但在急性呼吸性酸中毒时没有。在呼吸性酸中毒期间，较小的钙流出可能表明较少的净骨矿物质溶解，因此较少的缓冲液释放到培养基中。
During acute metabolic acidosis there is a net influx of protons into bone, decreasing the elevated proton concentration. Whether there is an influx of protons into bone during acute respiratory acidosis is not known. To determine the effect of respiratory acidosis on net proton flux (JH) relative to bone, we compared JH from neonatal mouse calvariae incubated for 3 h in medium acidified by an increase in PCO2 (respiratory acidosis) with that from calvariae incubated in medium acidified to the same extent by a decrease in bicarbonate concentration (metabolic acidosis). The initial medium pH with respiratory acidosis was not different from that with metabolic acidosis (7.108 .+-. 0.005 vs. 7.091 .+-. 0.007, respectively, P = NS). During respiratory acidosis there was no JH from bone relative to the medium (JH = 236 .+-. 93 neq .cntdot. bone-1 .cntdot. 3 h-1, P = NS vs. 0); however, during metabolic acidosis there was net proton influx from the medium into bone (JH = -703 .+-. 108, P < 0.05 vs 0, P < 0.001 vs. respiratory acidosis). There was less calcium efflux from bone during respiratory than during metabolic acidosis (JCa = 68 .+-. 6 nmol .cntdot. bone-1 .cntdot. 3 h-1 vs. 100 .+-. 9, respectively (P < 0.001). There is a net influx of protons into bone in vitro during acute metabolic, but not during acute respiratory, acidosis. The smaller calcium efflux during respiratory acidosis may indicate less net bone mineral dissolution and thus less buffer release into the medium.