Correlation between antibiotic susceptibilities and genotypes in Neisseria gonorrhoeae from different geographical origins: determinants monitoring by real-time PCR as a complementary tool for surveillance
Correlation between antibiotic susceptibilities and genotypes in Neisseria gonorrhoeae from different geographical origins: determinants monitoring by real-time PCR as a complementary tool for surveillance
复制标题
不同地理来源的淋病奈瑟菌抗生素敏感性与基因型之间的相关性：通过实时 PCR 进行的决定因素监测作为监测的补充工具
DOI：
10.1136/sti.2009.038000
复制
发表时间：
2009
影响因子：
3.6
通讯作者：
C. Goarant
中科院分区：
文献类型：
--
作者：
F. Vernel;E. Ratsima;B. Guillard;R. Goursaud;Camille Lethezer;S. Hem;F. Merien;C. Goarant
目的探讨淋病奈瑟菌（淋球菌）染色体耐药相关基因的监测是否与淋球菌表型相关，是否可作为淋球菌监测的补充工具。方法采用多重荧光定量PCR方法检测169份NG标本中的佩纳、mtrR、penB、ponA、gyrA和parC基因。用E试验测定青霉素和环丙沙星的最低抑菌浓度，用头孢硝肟纸片分析β-内酰胺酶的产生。结果共检查了169例NG，110例来自新喀里多尼亚，44例来自马达加斯加，15例来自柬埔寨。尽管测试的菌株数量的异质性，在不同的地理区域研究观察到的易感性趋势显示出良好的拟合与多基因基因型。此外，还发现了与特定地理多样性相关的特征：（1）在马达加斯加和柬埔寨，携带porB 1a等位基因的菌株的流行率很高，并显示青霉素敏感性降低（2）来自柬埔寨的菌株几乎全部耐药对青霉素和环丙沙星耐药率分别为11/15和14/15;和（3）在新喀里多尼亚发现与青霉素敏感性中度降低相关的新的penB和mtrR基因型（mtrR新型基因型在47%的中间型和14%的敏感型分离株中）。结论多重实时PCR检测与表型敏感性趋势具有良好的相关性，可成功用于前瞻性流行病学研究，特别是通过表征mtrR和penB决定簇在增加抗生素耐药性中的基本和互补作用。这些分子工具还可以为非活性菌株提供有用的替代监测工具。
Objective To determine in Neisseria gonorrhoeae (NG) isolates from different geographical areas whether monitoring of major determinants involved in chromosomal antimicrobial resistance correlated with phenotypes and could constitute complementary tools for surveillance. Methods Real-time multiplex PCR assays targeting penA, mtrR, penB, ponA, gyrA and parC determinants were applied to 169 NG extracts. Minimum inhibitory concentrations for penicillin and ciprofloxacin were determined by E tests, and β-lactamase production was analysed using nitrocefin discs. Results A total of 169 NGs were examined, 110 from New Caledonia, 44 from Madagascar and 15 from Cambodia. Despite the heterogeneity in the number of isolates tested, the susceptibility trends observed in the different geographic areas studied showed a good fit with the multigene genotypes. In addition, features related to a specific geographical diversity were found: (1) a high prevalence of strains harbouring the porB1a allele and showing reduced penicillin susceptibility in Madagascar and Cambodia (39% and 40% respectively); (2) almost all strains from Cambodia were resistant to the drugs tested (11/15 and 14/15 resistant to penicillin and ciprofloxacin respectively); and (3) identification of novel penB and mtrR genotypes associated with a moderately decreased penicillin susceptibility in New Caledonia (mtrR novel genotype in 47% of intermediate vs 14% of susceptible isolates). Conclusions Showing a good correlation with phenotypic trends of susceptibility, multiplex real-time PCR assays could be used successfully for prospective epidemiological studies notably by characterising mtrR and penB determinants for their fundamental and complementary roles in increasing the antibiotic resistance. These molecular tools could also provide useful alternative surveillance tools for non-viable strains.
影响因子：
4.9
作者：
Ito, M;Deguchi, T;Ezaki, T
通讯作者：
Ezaki, T
影响因子：
4.9
作者：
Ito, M;Yasuda, M;Deguchi, T
通讯作者：
Deguchi, T