Pathological Analysis of the Ruptured Vascular Wall of Hypoperfusion-induced Abdominal Aortic Aneurysm Animal Model
Pathological Analysis of the Ruptured Vascular Wall of Hypoperfusion-induced Abdominal Aortic Aneurysm Animal Model
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DOI：
10.5650/jos.ess16219
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发表时间：
2017-05-01
影响因子：
1.5
通讯作者：
Moriyama, Tatsuya
中科院分区：
文献类型：
--
作者：
Kugo, Hirona;Zaima, Nobuhiro;Moriyama, Tatsuya
腹主动脉瘤（AAA）是一种导致腹主动脉逐渐扩张的血管疾病，具有较高的破裂相关死亡率。然而，AAA破裂的机制仍不清楚。在我们前期的研究中，我们建立了一种新的自发性AAA破裂的AAA动物模型（低灌注诱导的AAA大鼠模型）。利用低灌注诱导的AAA大鼠模型，我们证明了血管壁脂肪细胞的异常外观与AAA破裂有关。然而，由于特别难以识别破裂点，因此尚未对破裂区域进行病理分析。在这项研究中，我们成功地从破裂点获得样品，并在低灌注诱导的AAA大鼠模型的血管壁破裂区域进行组织学分析。沿着血管壁AAA破裂区域观察到脂肪细胞。在脂肪细胞周围的区域，巨噬细胞浸润和基质金属蛋白酶2和9的蛋白水平显着增加，胶原蛋白阳性区域显着减少，与没有脂肪细胞的区域相比。低灌注诱导的AAA大鼠模型的AAA直径与血管壁中脂肪细胞的数量相关。另一方面，血清甘油三酯水平和血清总胆固醇水平与血管壁中脂肪细胞的数量无关。这些结果表明，局部脂肪细胞在血管壁中的积累，而不是血清脂质，在AAA破裂中具有重要作用。
Abdominal aortic aneurysm (AAA) is a vascular disease that results in the gradual dilation of the abdominal aorta and has a high rupture-related mortality rate. However, the mechanism of AAA rupture remains unknown. In our previous study, we established a novel AAA animal model (hypoperfusion-induced AAA rat model) with spontaneous AAA rupture. Using the hypoperfusion-induced AAA rat model, we demonstrated that the abnormal appearance of adipocytes in the vascular wall is associated with AAA rupture. However, pathological analysis of the rupture area has not been performed because it is particularly difficult to identify the rupture point. In this study, we succeeded in obtaining samples from the rupture point and performed a histological analysis of the ruptured area in the vascular wall in the hypoperfusion-induced AAA rat model. Adipocytes were observed along the AAA-ruptured area of the vascular wall. In the areas around the adipocytes, macrophage infiltration and protein levels of matrix metalloproteinases 2 and 9 were significantly increased and collagen-positive areas were significantly decreased, as compared with areas without adipocytes. The AAA diameter was correlated with the number of adipocytes in the vascular wall of the hypoperfusion-induced AAA rat model. On the other hand, serum triglyceride levels and serum total cholesterol levels were not correlated with the number of adipocytes in the vascular wall. These results suggest that local adipocyte accumulation in the vascular wall, not serum lipids, has an important role in AAA rupture.