A Kinetic Method for Serum 5’-Nucleotidase Using Stabilised Glutamate Dehydrogenase

A Kinetic Method for Serum 5’-Nucleotidase Using Stabilised Glutamate Dehydrogenase
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使用稳定谷氨酸脱氢酶测定血清 5’-核苷酸酶的动力学方法

DOI：
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发表时间：
1976
期刊：
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie
影响因子：
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通讯作者：
C. Arkesteijn
C. Arkesteijn
中科院分区：
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文献类型：
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作者：
C. Arkesteijn

文献摘要

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对动力学测定5‘-核苷酸酶(EC3.1.3.5)活性的方法进行了改进。重点研究了L亮氨酸对谷氨酸脱氢酶(EC 1.4.1.2)的稳定作用、NADH的最佳浓度以及内源氨的影响。试剂的其他成分的最佳浓度与文献中给出的最佳值进行了核对。测定正常值。该方法与比色参照法具有良好的相关性。
A modification of a kinetic determination of 5'-nucleotidase (EC 3.1.3.5) activity is described. Special attention has been paid to the stabilisation of glutamate dehydrogenase (EC 1.4.1.2) by L-leucine, optimal NADH concentration and the influence of endogeneous ammonia. The optimal concentrations of the other constituents of the reagent were checked with the optimal values given in the literature. Normal values were determined. The proposed method shows a good correlation with a colorimetric reference method.