The use of genomic in situ hybridization (GISH) to show transmission of recombinant chromosomes by a partially fertile bigeneric hybrid, Gasteria lutzii ×Aloe aristata (Aloaceae), to its progeny
The use of genomic in situ hybridization (GISH) to show transmission of recombinant chromosomes by a partially fertile bigeneric hybrid, Gasteria lutzii ×Aloe aristata (Aloaceae), to its progeny
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DOI：
10.1007/s004120050193
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发表时间：
1997-04
期刊：
影响因子：
1.6
通讯作者：
C. Takahashi;I. Leitch;A. Ryan;M. D. Bennett;P. Brandham
中科院分区：
文献类型：
--
作者：
C. Takahashi;I. Leitch;A. Ryan;M. D. Bennett;P. Brandham
利用基因组原位杂交技术（GISH）研究了植物中罕见的部分可育的芦荟（Aloe aristata）与绿腹菜（Gasteria lutzii）的双属杂种的亲本和后代（均为2n=2x=14）的体细胞染色体。GISH成功区分了F1杂种中的两个亲本基因组，并揭示了由F1传递到回交后代的染色体中的许多基因组重组。结果表明亲本基因组之间具有高水平的减数分裂相容性，尽管它们的大小相差20%。发生的频率高于预期的orcein染色的减数分裂在F1的分析。这种差异表明，终止可能发生在中期I之前或中期I期间，降低了明显的交叉频率，或者可能揭示了由眼睛分辨紧密分组的交叉的困难引起的低估。
Genomic in situ hybridization (GISH) was used to study somatic chromosomes of parental and progeny plants (all 2n=2x=14) of the bigeneric hybrid betweenGasteria lutziiandAloe aristata(Aloaceae), which is partially fertile, a rare occurrence in plants. GISH successfully distinguished between the two parental genomes in the F1 hybrid and revealed numerous genomic recombinations in chromosomes transmitted by the F1 to the back-cross progeny. The results indicate high levels of meiotic compatibility between the parental genomes, even though they differ in size by 20%. Recombination occurred at a frequency that was higher than that expected from the analysis of orcein-stained meiosis in the F1. The discrepancy suggests that terminalization may occur prior to or during metaphase I, reducing the apparent chiasma frequency, or possibly reveals an under-estimation caused by difficulties in resolving closely grouped chiasmata by eye.