Lung function, surfactant apoprotein content, and level of PEEP in prematurely delivered rabbits.
Lung function, surfactant apoprotein content, and level of PEEP in prematurely delivered rabbits.
复制标题
早产兔的肺功能、表面活性剂脱辅基蛋白含量和 PEEP 水平。
DOI：
10.1152/jappl.1994.77.4.1840
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Hawgood,S
中科院分区：
文献类型：
--
作者：
Ogawa,A;Brown,CL;Schlueter,MA;Benson,BJ;Clements,JA;Hawgood,S
为了研究表面活性剂载脂蛋白（SP-A, SP-B, SP-C）的体内活性，我们给早产的家兔幼仔注射了具有明确载脂蛋白组成的表面活性剂。给予含有双棕榈酰基磷脂酰胆碱和磷脂酰甘油的简单磷脂混合物，同时补充SP-B和SP-C或单独使用任一蛋白的家兔，在准静态压力-容积操作期间，通气和肺扩张时的肺顺应性明显高于盐水或脂质处理的对照组。对含有SP-B/C的表面活性剂的反应明显依赖于通气时使用的呼气末压力水平。当兔以4 cmH2O的呼气末正压通气时，SP-B/C处理兔的肺功能与表面活性剂处理兔的对照组无显著差异。在4 cmH2O的PEEP通气条件下，在含有SP-B/C的表面活性剂中添加SP-A并没有显著改善肺功能。当PEEP降低至1 cmH2O时，给予含有SP-B/C表面活性剂的幼兔的肺功能不再等同于表面活性剂处理的对照组。在低PEEP时，将SP-A添加到含有SP-B和SP-C的表面活性剂中可显著改善肺功能。当表面活性剂单独含有SP-B或SP-C时，SP-A没有明显的作用。我们得出的结论是，在包括适度PEEP的辅助通气下，SP-B和SP-C显著增强了简单磷脂混合物对早产家兔肺功能的影响。在低PEEP水平下，SP-B和SP-C对肺功能的影响明显降低，但可以通过添加SP-A来恢复。我们的研究结果与表面活性剂在体内功能中的协同蛋白-蛋白相互作用的存在一致，并表明对表面活性剂的反应将由通风策略和表面活性剂的组成决定。组成。
To study the in vivo activity of the surfactant apoproteins (SP-A, SP-B, SP-C), we administered surfactants with defined apoprotein compositions to prematurely delivered rabbit pups. Rabbits given simple phospholipid mixtures containing dipalmitoylphosphatidylcholine and phosphatidylglycerol supplemented with both SP-B and SP-C or either protein alone had significantly greater lung compliance during ventilation and lung expansion during a quasi-static pressure-volume maneuver than did saline-or lipid-treated controls. The response to the surfactants containing SP-B/C was markedly dependent on the level of end-expiratory pressure used during ventilation. When the rabbits were ventilated with a positive end-expiratory pressure (PEEP) of 4 cmH2O, lung function in the pups treated with SP-B/C was not significantly different from rabbit surfactant-treated controls. Addition of SP-A to the surfactants containing SP-B/C did not significantly further improve lung function if the pups were ventilated with a PEEP of 4 cmH2O. With a lower PEEP of 1 cmH2O, lung function in the pups given surfactants containing SP-B/C was no longer equivalent to the lung function of the rabbit surfactant-treated controls. At the lower PEEP, SP-A significantly improved lung function when it was added to surfactants containing SP-B and SP-C. No beneficial effect of SP-A was seen when the surfactant contained either SP-B or SP-C alone. We conclude that with assisted ventilation that includes a moderate level of PEEP, SP-B and SP-C significantly enhance the effect of a simple phospholipid mixture on the lung function of prematurely delivered rabbits. At lower levels of PEEP the effects of SP-B and SP-C on lung function are markedly reduced but can be restored by the addition of SP-A. Our results are consistent with the existence of cooperative protein-protein interactions in surfactant function in vivo and suggest that the response to a surfactant will be determined by both the ventilation strategy and the surfactant composition. composition.