Phosphorylated cAMP response element binding protein in the mouse brain after fear conditioning: relationship to Fos production
Phosphorylated cAMP response element binding protein in the mouse brain after fear conditioning: relationship to Fos production
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DOI：
10.1016/s0169-328x(01)00174-7
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发表时间：
2001-10-19
期刊：
影响因子：
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通讯作者：
Spiess, J
中科院分区：
文献类型：
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作者：
Stanciu, M;Radulovic, J;Spiess, J
在训练后立即开始，在 6 小时的时间间隔内，通过免疫组织化学方法监测小鼠大脑不同区域中由联想学习触发的 cAMP 反应元件结合蛋白 (pCREB) 的磷酸化。采用一项与情境相关的恐惧调节试验作为学习范例。培训包括情境暴露和随后的震惊。对照组由本地小鼠、单独暴露于环境的小鼠和暴露于环境中立即电击的小鼠组成。对于所有受过训练的小鼠，海马体、顶叶皮层和杏仁核中 CREB 磷酸化的时间进程表现出双相模式。早期阶段是训练后0到30分钟之间，后期阶段是训练后3到6分钟之间。暴露于环境后接受电击的动物以及暴露于立即电击的动物比仅接受环境的小鼠表现出显着更高的 pCREB 水平。在后期，暴露于环境的小鼠中 pCREB 水平最高，随后进行电击。观察到 CREB 磷酸化和 Fos 产生遵循不同的区域和刺激依赖性模式。提示pCREB增加的早期阶段可能与压力相关行为有关，而晚期阶段可能与记忆巩固有关。 (C) 2001 Elsevier Science BY 保留所有权利。
Phosphorylation of the cAMP response element binding protein (pCREB) triggered by associative learning was monitored immunohistochemically in different areas of the mouse brain during a 6-h interval, starting immediately after training. One trial context-dependent fear conditioning was employed as a learning paradigm. Training consisted of contextual exposure followed by shock. Control groups consisted of native mice, mice exposed to the context alone and mice exposed to an immediate shock in the context. For all trained mice, the time course of CREB phosphorylation in hippocampus, parietal cortex and amygdaloid nuclei exhibited a biphasic pattern. The early phase was between 0 and 30 min, and the late phase was between 3 and 6 It after training. The animals exposed to context followed by an electric shock, as well as those exposed to an immediate electric shock, exhibited significantly higher pCREB levels than the mice subjected to context alone. During the late phase, the pCREB levels were highest in the mice exposed to the context followed by shock, It was observed that CREB phosphorylation and Fos production followed different regional and stimulus-dependent patterns. It is suggested that the early phase of pCREB increase may be related to stress-related behaviors, whereas the late phase may rather relate to memory consolidation. (C) 2001 Elsevier Science BY All rights reserved.