Biodegradation of textile dye Reactive Blue 160 by Bacillus firmus (Bacillaceae: Bacillales) and non-target toxicity screening of their degraded products
Biodegradation of textile dye Reactive Blue 160 by Bacillus firmus (Bacillaceae: Bacillales) and non-target toxicity screening of their degraded products
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DOI：
10.1016/j.toxrep.2019.11.017
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发表时间：
2020-01-01
影响因子：
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通讯作者：
Padikasan, Indra Arulselvi
中科院分区：
文献类型：
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作者：
Barathi, Selvaraj;Karthik, Chinnannan;Padikasan, Indra Arulselvi
本研究旨在评价土著微生物对活性蓝160（RB 160）染料的脱色效果。从印度泰米尔纳德邦Noyyal河流域采集纺织染料工业污染土壤。通过16 SrRNA基因测序分析，确定该菌株为坚强芽孢杆菌（Bacillus firmus）。RB 160染料（500 μ g/ml）被B有效降解。对RB 160及其降解产物进行了firmus和毒理学分析。植物毒性试验表明，RB 160的降解产物被B降解为无毒。事务所用洋葱根细胞和人皮肤细胞株（CRL 1474）进行了毒性试验。毒力分析A. cepa和细胞系表明染料对根细胞具有毒性，RB 160的IC 50值显示对人皮肤细胞系具有毒性，而染料的降解产物的毒性中等。用RB 160和降解产物对斑马鱼胚胎的表型变形、存活率、孵化率和心率进行了毒性评价。然而，浓度为500 μ g/ml的RB 160降低了存活率、孵化率、心跳率和诱导的表型改变。其中，降解产物与染料相比在斑马鱼胚胎中表现出显著的发育。通过对RB 160的作用和B. firmus能有效降解RB 160，其降解产物对环境和水体无害。
The study was envisioned to evaluate the decolorization of Reactive Blue 160 (RB160) dye by using indigenous microbes. Contaminated soil from textile dye industry was collected from Noyyal river basin, Tamil Nadu, India. Potential dye degrading bacterial strain was recognized as Bacillus firmus by 16SrRNA gene sequencing analysis. RB160 dye (500 mu g/ml) was effectively degraded by B. firmus and toxicological analyses were performed with RB160 and their degraded product. Phytotoxicity revealed that degraded product of RB160 into non-toxic nature by B. firms. Toxicity assays were carried out on root cells of Allium cepa and human skin cell line (CRL 1474). Toxicity analysis of A. cepa and cell line signifies that dye exerts toxic cause on the root cells and IC50 values of RB160 showed toxic to human skin cell lines, while degradation products of the dye are moderately less in toxic. Zebrafish embryo toxicity also evaluated by RB160 and degraded product on phenotypic deformation, survival, hatching and heartbeat rate. However, RB160 with concentration of 500 mu g/ml decrease in the survival, hatching, heartbeat rate and induced phenotypic alterations. In which, degraded products exhibited significant development in zebrafish embryos as compared to dye. Based on the studies effects of RB160 and capability of B. firmus can effectively degrade RB160, and their degraded products were harmless to the environments and aquatic system.