Aspartate 141 Is the Fourth Ligand of the Oxygen-sensing [4Fe-4S]2+ Cluster of Bacillus subtilis Transcriptional Regulator Fnr
Aspartate 141 Is the Fourth Ligand of the Oxygen-sensing [4Fe-4S]2+ Cluster of Bacillus subtilis Transcriptional Regulator Fnr
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DOI：
10.1074/jbc.m110.191940
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发表时间：
2011-01-21
影响因子：
4.8
通讯作者：
Haertig, Elisabeth
中科院分区：
文献类型：
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作者：
Gruner, Ines;Fraedrich, Claudia;Haertig, Elisabeth
枯草芽孢杆菌氧化还原调节因子FNR控制厌氧代谢的基因，以响应低氧压。通过结合紫外可见光谱和穆斯堡尔光谱的遗传实验，发现了氧敏感[4Fe-4S](2+)团簇的一种不寻常的结构。ASP-141是除3个半胱氨酸残基外的第四个铁硫簇合物配体。Asp-141与Ala的交换取消了突变的FNR基因对FNR基因敲除菌株的体内功能互补和重组调节子FnrD141a的体外DNA结合。相反，用Cys取代Asp-141保留了[4Fe-4S](2+)的结构和调节功能。
The Bacillus subtilis redox regulator Fnr controls genes of the anaerobic metabolism in response to low oxygen tension. An unusual structure for the oxygen-sensing [4Fe-4S](2+) cluster was detected by a combination of genetic experiments with UV-visible and Mossbauer spectroscopy. Asp-141 was identified as the fourth iron-sulfur cluster ligand besides three Cys residues. Exchange of Asp-141 with Ala abolished functional in vivo complementation of an fnr knock-out strain by the mutagenized fnr gene and in vitro DNA binding of the recombinant regulator FnrD141A. In contrast, substitution of Asp-141 with Cys preserved [4Fe-4S](2+) structure and regulator function.