Separation of amino acid phenylthiohydantoins by high-performance liquid chromatography on phenylalkyl support.
Separation of amino acid phenylthiohydantoins by high-performance liquid chromatography on phenylalkyl support.
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在苯烷基载体上采用高效液相色谱法分离氨基酸苯硫基乙内酰脲。
DOI：
10.1016/0003-2697(80)90307-3
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发表时间：
1980
影响因子：
2.9
通讯作者：
S. Oroszlan
中科院分区：
文献类型：
--
作者：
L. Henderson;T. Copeland;S. Oroszlan
描述了一种高效液相色谱分析系统，用于分离，鉴定和定量苯硫代海因（PTH）衍生物的所有氨基酸通常在蛋白质中遇到。该系统在μBondapak苯烷基固定相上采用反相色谱。所有氨基酸的甲状旁腺素衍生物都可以用线性梯度洗脱在30分钟内进行鉴定和定量。该方法同样适用于pth衍生脱羧基氨基酸甲酯的分离。该方法的灵敏度可扩展到小于10pmol的范围内。
A high-performance liquid chromatographic analytical system is described for the separation, identification, and quantitation of phenylthiohydantoin (PTH) derivatives of all amino acids normally encountered in proteins. The system employs reverse-phase chromatography on a μBondapak phenylalkyl stationary phase. PTH derivatives of all amino acids can be identified and quantitated in a single 30-min analysis using linear gradient elution. The method can be applied equally well to the separation of methyl esters of the PTH-derivatized decarboxylic amino acids. The sensitivity of the method extends into the range of less than 10 pmol.