Molecular cloning of htra2-beta-1 and htra2-beta-2, two human homologs of tra-2 generated by alternative splicing
Molecular cloning of htra2-beta-1 and htra2-beta-2, two human homologs of tra-2 generated by alternative splicing
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DOI：
10.1089/dna.1997.16.679
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发表时间：
1997-06-01
影响因子：
3.1
通讯作者：
Stamm, S
中科院分区：
文献类型：
--
作者：
Beil, B;Screaton, G;Stamm, S
进行酵母双杂交筛选以寻找参与前体 mRNA 剪接的新因子。使用SC35作为诱饵，我们分离出与果蝇transformer-2（TRA-2）蛋白具有高度同源性的人类cDNA，该cDNA被命名为htra2-beta1，htra2-beta1是一种核蛋白，以斑点模式与SC35共定位，它与在酵母中测试的几种SR蛋白相互作用。第二种形式称为 htra2-beta2，是通过选择性剪接产生的。该同工型产生没有 SR 结构域的截短蛋白质。两种同工型均匀分布在成年大鼠组织中。在刺激原代人 T 细胞和原代大鼠脾细胞培养物后，这两种异构体的比例发生变化，表明选择性剪接参与了 htra2-β 活性的调节。
A yeast two-hybrid screen was performed to find new factors involved in pre-mRNA splicing. Using SC35 as a bait, we isolated a human cDNA bearing high homology to the Drosophila transformer-2 (TRA-2) protein, This cDNA was named htra2-beta1, htra2-beta1 is a nuclear protein that colocalizes with SC35 in a speckled pattern, It interacts with several SR proteins tested in yeast. A second form named htra2-beta2 is generated by alternative splicing, This isoform gives rise to a truncated protein without an SR domain, Both isoforms are evenly distributed throughout adult rat tissue. The ratio of these two isoforms changes after stimulation of primary human T-cell and primary rat spleen cell cultures, indicating that alternative splicing is involved in regulation of htra2-beta activity.