Myxococcus xanthus sasS encodes a sensor histidine kinase required for early developmental gene expression.
Myxococcus xanthus sasS encodes a sensor histidine kinase required for early developmental gene expression.
复制标题
黄粘球菌 sasS 编码早期发育基因表达所需的传感器组氨酸激酶。
DOI：
10.1128/jb.179.24.7759-7767.1997
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发表时间：
1997
影响因子：
3.2
通讯作者：
Kaplan,HB
中科院分区：
文献类型：
--
作者：
Yang,C;Kaplan,HB
Initiation of Myxococcus xanthus multicellular development requires integration of information concerning the cells' nutrient status and density. A gain-of-function mutation, sasB7, that bypasses both the starvation and high cell density requirements for developmental expression of the 4521 reporter gene, maps to the sasS gene. The wild-type sasS gene was cloned and sequenced. This gene is predicted to encode a sensor histidine protein kinase that appears to be a key element in the transduction of starvation and cell density inputs. The sasS null mutants express 4521 at a basal level, form defective fruiting bodies, and exhibit reduced sporulation efficiencies. These data indicate that the wild-type sasS gene product functions as a positive regulator of 4521 expression and participates in M. xanthus development. The N terminus of SasS is predicted to contain two transmembrane domains that would locate the protein to the cytoplasmic membrane. The sasB7 mutation, an E139K missense mutation, maps to the predicted N-terminal periplasmic region. The C terminus of SasS contains all of the conserved residues typical of the sensor histidine protein kinases. SasS is predicted to be the sensor protein in a two-component system that integrates information required for M. xanthus developmental gene expression.
