Effects of a hyaluronate-carboxymethylcellulose membrane (Seprafilm) on human polymorphonuclear neutrophil functions.
Effects of a hyaluronate-carboxymethylcellulose membrane (Seprafilm) on human polymorphonuclear neutrophil functions.
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DOI：
10.1016/j.jss.2008.01.011
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发表时间：
2008-10
期刊：
影响因子：
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通讯作者：
K. Otake;K. Uchida;S. Yoshiyama;M. Inoue;Y. Okita;Hideki Watanabe;Y. Inoue;Y. Mohri;C. Miki;M. Kusunoki
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文献类型：
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作者：
K. Otake;K. Uchida;S. Yoshiyama;M. Inoue;Y. Okita;Hideki Watanabe;Y. Inoue;Y. Mohri;C. Miki;M. Kusunoki
SEPRAFILM（Genzyme Corp.，剑桥，MA）可安全有效地减少腹部和盆腔手术中的粘连形成。然而，Seprafilm与术后并发症（包括腹腔内脓肿形成和腹腔内炎症）之间的关系仍存在争议。多形核中性粒细胞（polymorphisms neutrophil，PMNs）在腹腔内炎症、感染和脓肿形成过程中起着至关重要的作用，并且PMNs在针对广泛病原体的非特异性宿主防御中充当第一线。本研究评价Seprafilm本身对人中性粒细胞在体外实验模型中的影响。材料和方法人中性粒细胞从14名健康志愿者的血液样品中分离。在体外炎症和内毒素刺激实验模型中，通过检测它们的吞噬作用、凋亡/坏死率、细胞因子产生和有无Seprafilm的PMN弹性蛋白酶释放来评估分离细胞的功能。或在有和没有Seprafilm的测定之间与细菌共培养后PMN-弹性蛋白酶释放到上清液中。在涉及肿瘤坏死因子-α和脂多糖的PMN刺激模型中，使用和不使用Seprafilm的试验之间细胞因子产生无显著差异。此外，一个类似的实验测量细胞因子的产生在溶解良好的Seprafilm的存在下产生相应的results.CONCLUSIONSOUR的结果表明Seprafilm本身不影响功能的分离的人中性粒细胞在体外实验模型的炎症和内毒素刺激。
BACKGROUNDSeprafilm (Genzyme Corp., Cambridge, MA) is safe and effective for reducing adhesion formation in abdominal and pelvic surgery. However, the relationships between Seprafilm and postoperative complications, including intra-abdominal abscess formation and intra-abdominal inflammation, remain controversial. Polymorphonuclear neutrophils (PMNs) play crucial roles in the processes of intra-abdominal inflammation, infection, and abscess formation, and PMNs act as the first line in nonspecific host defense against a wide range of pathogens. The present study evaluates the effect of Seprafilm itself on human PMNs in experimental models in vitro.MATERIALS AND METHODSHuman PMNs were isolated from blood samples obtained from 14 healthy volunteers. The functions of the isolated cells were assessed by examining their phagocytosis, apoptosis/necrosis rates, cytokine production, and PMN-elastase release with and without Seprafilm in an in vitro experimental model of inflammation and endotoxin-stimulation.RESULTSThere were no significant differences in the PMN phagocytosis, apoptosis/necrosis rates, cytokine production, or PMN-elastase release into the supernatant after coculture with bacteria between assays with and without Seprafilm. In the PMN stimulation models involving tumor necrosis factor-alpha and lipopolysaccharide, there were no significant differences in cytokine production between assays with and without Seprafilm. Furthermore, a similar experiment measuring cytokine production in the presence of well-dissolved Seprafilm produced corresponding results.CONCLUSIONSOur results suggest Seprafilm itself does not affect the functions of isolated human PMNs in in vitro experimental models of inflammation and endotoxin-stimulation.