Highly feasible procedure for laparoscopic transplantation of cell sheets under pneumoperitoneum in porcine model
Highly feasible procedure for laparoscopic transplantation of cell sheets under pneumoperitoneum in porcine model
复制标题
猪模型气腹下腹腔镜细胞片移植的高度可行的程序
DOI：
10.1007/s00464-021-08708-3
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Eguchi Susumu
中科院分区：
文献类型：
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作者：
Yamaguchi Shun;Kanetaka Kengo;Maruya Yasuhiro;Higashi Miki;Kobayashi Shinichiro;Hashiguchi Keiichi;Oohashi Fumiya;Sakai Yusuke;Nakao Kazuhiko;Eguchi Susumu
细胞片层技术是再生医学中最成功的方法之一。细胞片的各种应用已经在几个临床领域的首次人体研究中引入。当将细胞片移植到内部器官中时，由于难以处理该片，因此需要相对大的切口用于输送。我们开发了一种腹腔镜下交付程序安全，方便移植的细胞片在猪model.Methodspneumocarcinum建立与CO2充气。首先，为了增加处理过程中的强度，将纤维蛋白喷涂到细胞片的表面上，然后将成肌细胞片置于新开发的载体上。用腹腔镜镊子夹住床单，通过腹腔镜端口插入腹腔。然后将成肌细胞片应用于肝脏、结肠、小肠和胃的表面，并测量手术时间。在移植后三天，进行组织病理学检查以确认片材的植入。的功能和植入物也进行了分析，在十二指肠内镜粘膜下剥离术（ESD）model.ResultsThe纤维蛋白处理成肌细胞片能够管理与传统的腹腔镜钳没有打破。尽管通过腹腔镜端口时气压发生了剧烈变化，但床单没有明显损坏。移植手术时间在移植部位之间没有显著差异。组织病理学检查显示，在每个移植部位的薄层，结蛋白阳性细胞。通过ESD后的移植，植入的成肌细胞片有效地防止了延迟穿孔。结论我们的手术很简单，该系统包括由医用硅胶制成的载体、市售纤维蛋白胶和传统的腹腔镜镊子。我们的程序是一个强大的工具，腹腔镜细胞片移植。
IntroductionCell sheet technology is one of the most successful methodologies in regenerative medicine. Various applications of cell sheets have been introduced in first-in-human studies in several clinical fields. When transplanting a cell sheet into internal organs, a relatively large incision is required for delivery due to difficulty handling the sheet. We developed a laparoscopic delivery procedure for safe and easy transplantation of cell sheets in a porcine model.MethodsPneumoperitoneum was established by inflation with CO2. First, to increase the strength during handling, fibrin was sprayed onto the surface of the cell sheet, and then a myoblast sheet was placed onto the newly developed carrier. The sheets were pinched with laparoscopic forceps to insert into the abdominal cavity through the laparoscopic port. Myoblast sheets were then applied to the surface of the liver, colon, small intestine, and stomach, and procedure times were measured. At three days post transplantation, a histopathological examination was performed to confirm engraftment of the sheet. The function and engraftment were also analyzed in a duodenal endoscopic submucosal dissection (ESD) model.ResultsThe fibrin-processed myoblast sheet was able to be managed with conventional laparoscopic forceps without breaking. Despite the drastic change in air pressure by passing through the laparoscopic port, the sheets suffered no apparent damage. The transplantation procedure times did not markedly differ among transplant sites. A histopathological examination revealed thin-layered, desmin-positive cells at each transplant site. With transplantation following ESD, the engrafted myoblast sheets effectively prevented delayed perforation.ConclusionsOur procedure is simple, and the system involves a carrier made of medically fit silicon, commercially available fibrin glue and conventional laparoscopic forceps. Our procedure is a powerful tool for laparoscopical cell sheet transplantation.