Potent and Broad-Spectrum Antimicrobial Activity of CXCL14 Suggests an Immediate Role in Skin Infections
Potent and Broad-Spectrum Antimicrobial Activity of CXCL14 Suggests an Immediate Role in Skin Infections
复制标题
DOI：
10.4049/jimmunol.182.1.507
复制
发表时间：
2009-01-01
影响因子：
4.4
通讯作者：
Wolf, Marlene
中科院分区：
文献类型：
--
作者：
Maerki, Christa;Meuter, Simone;Wolf, Marlene
皮肤经常暴露于肠道微生物和病原微生物。最外层皮肤的角质层采用不同的工具，如苛刻的生长条件和众多的抗菌肽（AMP），以区分有益的皮肤微生物菌群和有害细菌。皮肤如何处理由于微损伤而进入皮肤活的部分的微生物是不明确的。在这项研究中，我们报告说，趋化因子CXCL 14是一种广谱AMP与皮肤革兰氏阳性菌和白色念珠菌以及革兰氏阴性肠杆菌大肠杆菌的杀伤活性。基于两个单独的细菌杀伤测定，CXCL 14与其他测试的AMP（包括人β-防御素和趋化因子CCL 20）相比是有利的。增加盐浓度和皮肤典型的pH条件并没有废除其AMP功能。这种新型AMP在健康人类皮肤的表皮和真皮中高度丰富，但在炎症和疾病条件下下调。我们认为CXCL 14在感染的最早阶段，即炎症建立之前就能对抗细菌，因此在抗菌免疫中发挥了独特的作用。免疫学杂志，2009，182：507-514.
The skin is constantly exposed to commensal microflora and pathogenic microbes. The stratum corneum of the outermost skin layer employs distinct tools such as harsh growth conditions and numerous antimicrobial peptides (AMPs) to discriminate between beneficial cutaneous microflora and harmful bacteria. How the skin deals with microbes that have gained access to the live part of the skin as a result of microinjuries is ill defined. In this study, we report that the chemokine CXCL14 is a broad-spectrum AMP with killing activity for cutaneous Gram-positive bacteria and Candida albicans as well as the Gram-negative enterobacterium Escherichia coli. Based on two separate bacteria-killing assays, CXCL14 compares favorably with other tested AMPs, including human beta-defensin and the chemokine CCL20. Increased salt concentrations and skin-typical pH conditions did not abrogate its AMP function. This novel AMP is highly abundant in the epidermis and dermis of healthy human skin but is down-modulated under conditions of inflammation and disease. We propose that CXCL14 fights bacteria at the earliest stage of infection, well before the establishment of inflammation, and thus fulfills a unique role in antimicrobial immunity. The Journal of Immunology, 2009, 182: 507-514.