Alkaline phosphatase-catalyzed silver deposition for electrochemical detection
Alkaline phosphatase-catalyzed silver deposition for electrochemical detection
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DOI：
10.1021/ac070624o
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发表时间：
2007-07-15
影响因子：
7.4
通讯作者：
Costa-Garcia, Agustin
中科院分区：
文献类型：
--
作者：
Fanjul-Bolado, Pablo;Hernandez-Santos, David;Costa-Garcia, Agustin
碱性磷酸酶（AP）是开发ELISA、免疫传感器、DNA杂交测定等最常用的酶标记之一。这种酶催化底物脱磷酸化为可检测产物，通常通过光学或电化学测量进行定量。这项工作是基于一种底物（3-吲哚酚磷酸盐），该底物产生一种化合物，该化合物能够将溶液中的银离子还原成金属存款，该金属存款位于酶标记AP附着的地方。将沉积的银电化学剥离到溶液中并通过阳极剥离伏安法测量。它的应用链霉亲和素修饰的丝网印刷碳电极上的酶基因传感器检测的毒力核酸决定簇的自溶素基因，专门存在于人类病原体肺炎链球菌的基因组，描述。与靛蓝胭脂红的直接伏安法检测相比，银离子的阳极溶出伏安法的灵敏度提高了14倍。
Alkaline phosphatase (AP) is one of the most used enzymatic labels for the development of ELISAs, immunosensors, DNA hybridization assays, etc. This enzyme catalyzes the dephosphorylation of a substrate into a detectable product usually quantified by optical or electrochemical measurements. This work is based on a substrate (3-indoxyl phosphate) that produces a compound able to reduce silver ions in solution into a metallic deposit, which is localized where the enzymatic label AP is attached. The deposited silver is electrochemically stripped into solution and measured by anodic stripping voltammetry. Its application to an enzymatic genosensor on streptavidin-modified screen-printed carbon electrodes for the detection of virulence nucleic acid determinants of autolysin gene, exclusively present on the genome of the human pathogen Streptococcus pneumoniae, is described. Compared with the direct voltammetric detection of indigo carmine, the anodic stripping voltammetry of silver ions is 14-fold more sensitive.